RAR alpha/NR1B1 Antibody

Novus Biologicals | Catalog # NB300-969

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RAR alpha/NR1B1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SPSLSPSSHRSSPATQSP corresponding to C-Terminus according to NP_000955.1, NP_001138773.1, NP_001019980.1, NP_001138774.1.

Specificity

This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. Reported variants NP_000955.1 and NP_001138773.1 represent identical proteins.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RAR alpha/NR1B1 Antibody

Western Blot: RAR alpha/NR1B1 Antibody [NB300-969]

Western Blot: RAR alpha/NR1B1 Antibody [NB300-969]

Western Blot: RAR alpha/NR1B1 Antibody [NB300-969] - Staining of Human Breast (A) and Breast cancer (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for RAR alpha/NR1B1 Antibody

Application
Recommended Usage

Peptide ELISA

detection limit 1:8000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx 55kDa band observed in Human Breast and Breast cancer lysates (calculated MW of 50.8kDa according to NP_000955.1and NP_001019980.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAR alpha/NR1B1

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma.

Long Name

Retinoic Acid Receptor, alpha

Alternate Names

NR1B1, RARA

Entrez Gene IDs

5914 (Human)

Gene Symbol

RARA

UniProt

NP_000955.1 (Human)

Additional RAR alpha/NR1B1 Products

Product Documents for RAR alpha/NR1B1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RAR alpha/NR1B1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAR alpha/NR1B1 Antibody

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