RAR beta/NR1B2 Antibody (336) - Azide Free

Novus Biologicals | Catalog # NB200-323

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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Canine (100%), Guinea Pig (100%), Mouse (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 336

Format

Azide Free
View all RAR beta/NR1B2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P10826

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 18272600). It is anticipated that the antibody will also work with canine, guinea pig, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~48 kDa RAR beta/NR1B2 isotype.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAR beta/NR1B2 Antibody (336) - Azide Free

Western Blot: RAR beta/NR1B2 Antibody (336) [NB200-323]

Western Blot: RAR beta/NR1B2 Antibody (336) [NB200-323]

Western Blot: Retinoic Acid Receptor beta Antibody (336) [NB200-323] - Western blot of Rat hippocampal lysate showing specific immunolabeling of the ~ 48k RAR-B isotype.

Applications for RAR beta/NR1B2 Antibody (336) - Azide Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
By Western blot analysis a ~48 kDa band can be seen using rat brain hippocampal homogenate. WB was tested in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 1% Tween-20) containing 5% nonfat milk and 0.05% NaN3.

Reviewed Applications

Read 2 reviews rated 3 using NB200-323 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAR beta/NR1B2

Retinoids can reverse various premalignant lesions as well as prevent some second primary tumors. The effects of retinoids are regulated by retinoic acid receptors (RAR) and retinoid X receptors, which act as ligand-activated transcription factors. Ligand-dependent transcriptional activation of RARs is a multistep process that ends with the formation of a multimeric co-activator complex on regulated promoters (1,2). Loss of RAR-beta expression and the accumulation of p53 and Ki67 proteins may help in the early identification of esophageal cancer in high-risk populations (3).

Long Name

Retinoic Acid Receptor, beta

Alternate Names

HAP, NR1B2, RARB, RRB2

Entrez Gene IDs

5915 (Human); 24706 (Rat)

Gene Symbol

RARB

UniProt

P10826 (Human)

Additional RAR beta/NR1B2 Products

Product Documents for RAR beta/NR1B2 Antibody (336) - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RAR beta/NR1B2 Antibody (336) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAR beta/NR1B2 Antibody (336) - Azide Free

Customer Reviews for RAR beta/NR1B2 Antibody (336) - Azide Free (2)

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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Overexpressing COS cells
    Species: Other
    Verified Customer | Posted 04/20/2015
    Western Blotting of COS cells overexpressing RARalpha2, RARbeta2, or RARgamma2
    RAR beta/NR1B2 Antibody (336) - Azide Free NB200-323
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T and HePG2 cell lysate
    Species: Human
    Verified Customer | Posted 09/05/2014
    WB for RARbeta in 293T and HePG2 cells
    RAR beta/NR1B2 Antibody (336) - Azide Free NB200-323

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FAQs for RAR beta/NR1B2 Antibody (336) - Azide Free

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  • Q: I am planning to perform IHC staining of RARB on FFPE samples. I have found the reference "J Cutan Pathol 2009;36; 1141-1145" used an antibody from your company. Would you let me know any other references using the antibody for IHC staining of RARB?

    A:

    Unfortunately we are not aware of any other publication wherein our Retinoic Acid Receptor beta antibodies were cited for their use in IHC application. From our sales records, it looks like the researcher used catalog number NB200-323 in the publication you mentioned. Retinoic Acid Receptor beta antibody NB200-323 is our best selling product among this category and it has also been cited for its use in WB application by Yang et al. in Am J Physiol Renal Physiol. 2008 Jun;294(6):F1433-40. We have at least 7 primary antibody products to Retinoic Acid Receptor beta that have been validated for IHC-P application.. We stand behind the quality of our products and offer 100% guarantee for the applications/species mentioned on the datasheets. We are always there to help if you come up with any difficulties and yes, we provide replacements/refunds after troubleshooting a problem, if you do not get your desired outcome! Therefore, please let us know if you have any specific questions or concerns on the IHC-P application of our Retinoic Acid Receptor beta antibodies. You may contact us at technical@novusbio.com.

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