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Rat CD3+ T Cell Enrichment Column, Small, Pack of 25

Catalog #: RTCC-25
5 Citations Datasheet / COA / SDS

Discontinued Product

RTCC-25 has been discontinued.
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R&D Systems Cell Enrichment Column Kits: Antibody-coated Silica Beads
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Rat CD3+ T Cell Enrichment Column, Small, Pack of 25 Summary

T Cell Enrichment Columns

T Cell Enrichment Columns Assay Principle
View Full Assay Principle

Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.

CD3+ is a universal marker for T cells. Total CD3+ cell recovery ranges between 30% and 50% of initial CD3+ cells loaded. The purity of recovered cells ranges from 90% to 94%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.

Features

  • Available for human, mouse, and rat cells
  • Purifies CD3+ T cells to 90 – 94%
  • Based upon gravitational flow
  • Simple procedure
  • No specialized equipment required

Kit Contents

  • Cell Selection Column
  • 10X Column Buffer

Specifications

Source
N/A
Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Rat

Product Datasheets

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Product Datasheet
COA
SDS

Background: CD3

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Entrez Gene IDs
916 (Human); 12501 (Mouse)
Alternate Names
CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Citations for Rat CD3+ T Cell Enrichment Column, Small, Pack of 25

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Induced autoimmunity to heat shock proteins elicits glaucomatous loss of retinal ganglion cell neurons via activated T-cell-derived fas-ligand.
    Authors: Wax MB, Tezel G, Yang J, Peng G, Patil RV, Agarwal N, Sappington RM, Calkins DJ
    J. Neurosci., 2008-11-12;28(46):12085-96.  2008-11-12
  2. Blockade of the interleukin-21/interleukin-21 receptor pathway ameliorates disease in animal models of rheumatoid arthritis.
    Authors: Young DA, Hegen M, Ma HL, Whitters MJ, Albert LM, Lowe L, Senices M, Wu PW, Sibley B, Leathurby Y, Brown TP, Nickerson-Nutter C, Keith JC, Collins M
    Arthritis Rheum., 2007-04-01;56(4):1152-63.  2007-04-01
  3. Comparative analysis of the fate of donor dendritic cells and B cells and their influence on alloreactive T cell responses under tacrolimus immunosuppression.
    Authors: Azhipa O, Kimizuka K, Nakao A, Toyokawa H, Okuda T, Neto JS, Alber SM, Kaizu T, Thomson AW, Demetris AJ, Murase N
    Clin. Immunol., 2005-02-01;114(2):199-209.  2005-02-01
  4. Phylogenetic disparity influences the predominance of direct over indirect pathway of antigen presentation in islet xenotransplantation.
    Authors: Andres A, Toso C, Morel P, Bosco D, Bucher P, Oberholzer J, Mathe Z, Mai G, Wekerle T, Berney T, Buhler LH
    Transplant. Proc., 2005-01-01;37(1):463-5.  2005-01-01
  5. Efficacy of peripheral tolerance induced by dendritic cells is dependent on route of delivery.
    Authors: Zhang QH, Link H, Xiao BG
    J. Autoimmun., 2004-08-01;23(1):37-43.  2004-08-01

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