Detection of Rat IgGPrimary Antibody by Flow Cytometry
RAW 264.7 mouse monocyte/macrophage cell line wasstained with Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog #MAB7774,filled histogram) or isotype control antibody (open histogram), followedby Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B)at 10 uL/106 cells. Tofacilitate intracellular staining, cells were fixed with paraformaldehyde andpermeabilized with saponin.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
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