Rat F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

R&D Systems | Catalog # F0104B

R&D Systems
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Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rat IgG heavy and light chains

Specificity

Rat IgG specific IgG was purified by rat IgG affinity chromatography.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

Detection of Rat IgG Primary Antibody by Flow Cytometry

Detection of Rat IgG Primary Antibody by Flow Cytometry

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774, filled histogram) or isotype control antibody (open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B) at 10 uL/106 cells. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Rat F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of rabbit origin, and is of IgG class. The recommended concentration is 10 μL/106 cells.

Reviewed Applications

Read 2 reviews rated 4.5 using F0104B in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Rat F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

For research use only

Citations for Rat F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody

Customer Reviews for Rat F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody
    Name: Liang Lin
    Application: Flow Cytometry
    Sample Tested: CELL LINE and CH-1 mouse B cell lymphoma cell line
    Species: Mouse
    Verified Customer | Posted 09/30/2020
    Rat F(ab)2 IgG (H+L) Fluorescein‑conjugated Antibody F0104B
  • Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 09/01/2016

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