Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat IgG Primary Antibody by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774, filled histogram) or isotype control antibody (open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B) at 10 uL/106 cells. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Citation for Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The N-terminal region of the atypical chemokine receptor ACKR2 is a key determinant of ligand binding.
Authors: Hewit, Kay D, Fraser, Alasdair, Nibbs, Robert J, Graham, Gerard J
J Biol Chem, 2014-03-18;289(18):12330-42.
Sample Types: Whole Cells
Applications: Flow Cytometry
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