Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of Rat IgG Primary Antibody by Flow Cytometry
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Product Details
Citations (1)
Supplemental Products
Reviews (2)

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody Summary

Species Reactivity
Rat IgG specific IgG was purified by rat IgG affinity chromatography.
Polyclonal Goat IgG
Antigen Affinity-purified
Rat IgG heavy and light chains
Supplied in a saline solution containing BSA and Sodium Azide.
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Rat IgG Primary Antibody by Flow Cytometry View Larger

Detection of Rat IgG Primary Antibody by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774, filled histogram) or isotype control antibody (open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B) at 10 uL/106 cells. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citation for Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The N-terminal region of the atypical chemokine receptor ACKR2 is a key determinant of ligand binding.
    Authors: Hewit, Kay D, Fraser, Alasdair, Nibbs, Robert J, Graham, Gerard J
    J Biol Chem, 2014-03-18;289(18):12330-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Reviews for Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody
By Liang Lin on 09/30/2020
Application: Flow Sample Tested: CELL LINE,CH-1 mouse B cell lymphoma cell line Species: Mouse

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody
By Anonymous on 09/01/2016
Application: Flow Sample Tested: Bone marrow cells Species: Mouse