Rat ICAM-1/CD54 Fluorescein-conjugated Antibody Summary
Accession # Q00238
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of ICAM‑1/CD54 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Rat ICAM-1/CD54 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5831F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 ( alpha L/ beta 2 or CD11a/CD18) and Mac-1 ( alpha M/ beta 2 or CD11b/CD18). ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Within the extracellular domain, rat ICAM-1 shares 53% and 78% amino acid sequence identity with human and mouse ICAM-1, respectively. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.
- Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
- Siu, G. et al. (1989) J. Immunol. 143:3813.
- Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Citation for Rat ICAM-1/CD54 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Nicotinamide mononucleotide (NMN) supplementation rescues cerebromicrovascular endothelial function and neurovascular coupling responses and improves cognitive function in aged mice
Authors: S Tarantini, MN Valcarcel-, P Toth, A Yabluchans, Z Tucsek, T Kiss, P Hertelendy, M Kinter, P Ballabh, Z Süle, E Farkas, JA Baur, DA Sinclair, A Csiszar, Z Ungvari
Redox Biol, 2019;24(0):101192.
Sample Types: Whole Cells
Applications: Flow Cytometry
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