Rat M-CSF Antibody (427406)

R&D Systems | Catalog # MAB5364

R&D Systems
Discontinued Product
MAB5364 has been discontinued. View all M-CSF products.

Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 427406
View all M-CSF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat M‑CSF
Glu33-Glu261
Accession # Q8JZQ0

Specificity

Detects rat M-CSF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse M‑CSF and recombinant human M‑CSF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Rat M-CSF Antibody (427406)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat M-CSF

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha −helical-bundle cytokine that is essential for survival, proliferation and differentiation of macrophages and osteoclast progenitors. M-CSF is produced by fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. Full-length rat M-CSF transcripts encode a 534 amino acid (aa) type I transmembrane mature protein. In humans this encodes a 70 kDa protein that forms covalent homodimers. Human 22 kDa, 43 kDa and proteoglycan-modified 200 kDa secreted forms and 34 kDa transmembrane forms may also occur in rats. Mature rat M-CSF shares 89% and 74% aa identity with mouse and human M-CSF, respectively, within the first 229 aa.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

UniProt

Q8JZQ0

Additional M-CSF Products

Product Documents for Rat M-CSF Antibody (427406)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat M-CSF Antibody (427406)

For research use only

Citations for Rat M-CSF Antibody (427406)

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