Rat MCAM/CD146 Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry.
1 Image
Product Details
Supplemental Products

Rat MCAM/CD146 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Detects rat MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed.
Monoclonal Mouse IgG2A Clone # 404722
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
Accession # Q9EPF2
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of MCAM/CD146 antibody in PC-12 Rat Cell Line antibody by Flow Cytometry. View Larger

Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB3250T, filled histogram) or isotype control antibody (Catalog # IC003T, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

Melanomia Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.

Long Name
Melanoma Cell Adhesion Molecule
Entrez Gene IDs
4162 (Human); 84004 (Mouse); 78967 (Rat)
Alternate Names
CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

Product Datasheets


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Rat MCAM/CD146 Alexa Fluor® 594-conjugated Antibody

There are currently no reviews for this product. Be the first to review Rat MCAM/CD146 Alexa Fluor® 594-conjugated Antibody and earn rewards!

Have you used Rat MCAM/CD146 Alexa Fluor® 594-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review