Rat MCAM/CD146 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry.
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Product Details
Citations (2)
Supplemental Products

Rat MCAM/CD146 PE-conjugated Antibody Summary

Species Reactivity
Detects rat MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed.
Monoclonal Mouse IgG2A Clone # 404722
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
Accession # Q9EPF2
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MCAM/CD146 antibody in PC-12 Rat Cell Line antibody by Flow Cytometry. View Larger

Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 PE-conjugated Monoclonal Antibody (Catalog # FAB3250P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

Melanoma Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is an Ig-superfamily adhesion molecule that is expressed on endothelial cells, neurons and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.

Long Name
Melanoma Cell Adhesion Molecule
Entrez Gene IDs
4162 (Human); 84004 (Mouse); 78967 (Rat)
Alternate Names
CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin

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Citations for Rat MCAM/CD146 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Nicotinamide mononucleotide (NMN) supplementation rescues cerebromicrovascular endothelial function and neurovascular coupling responses and improves cognitive function in aged mice
    Authors: S Tarantini, MN Valcarcel-, P Toth, A Yabluchans, Z Tucsek, T Kiss, P Hertelendy, M Kinter, P Ballabh, Z Süle, E Farkas, JA Baur, DA Sinclair, A Csiszar, Z Ungvari
    Redox Biol, 2019;24(0):101192.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Systemic administration of erythropoietin inhibits retinopathy in RCS rats.
    Authors: Shen W, Chung S, Irhimeh M, Li S, Lee S, Gillies M
    PLoS ONE, 2014;9(8):e104759.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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