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Rat Total MMP-9 Quantikine ELISA Kit

Catalog #: RMP900
11 Citations 3 Reviews Datasheet / COA / SDS
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RMP900
Rat Total MMP-9 ELISA Standard Curve
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Product Details
Procedure
Citations (11)
FAQs
Supplemental Products
Reviews (3)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Rat Total MMP-9 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor Heparin Plasma (10 uL)
Sensitivity
0.028 ng/mL
Assay Range
0.2 - 10 ng/mL
Specificity
Natural and recombinant rat MMP-9 (Pro-, active, and TIMP-complexed)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat Total MMP-9 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total rat MMP-9 (Pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat MMP-9 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obtained using natural rat MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat MMP-9.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.39 1.22 5.27 0.51 1.18 3.55
Standard Deviation 0.027 0.068 0.238 0.035 0.046 0.153
CV% 6.9 5.6 4.5 6.9 3.9 4.3

Recovery

The recovery of rat MMP-9 spiked into various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 94 87-107
Platelet-poor Heparin Plasma (n=4) 92 80-103
Serum (n=4) 97 90-115

Linearity

To assess the linearity of the assay, samples containing high concentrations of rat MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Rat Total MMP-9 ELISA Linearity

Scientific Data

N/A Rat Total MMP-9 ELISA Standard Curve View Larger

Rat Total MMP-9 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 9
Entrez Gene IDs:
4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names:
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Rat Total MMP-9 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. A novel rat model of chronic subdural hematoma: Induction of inflammation and angiogenesis in the subdural space mimicking human-like features of progressively expanding hematoma
    Authors: X Xu, D Wang, Z Han, B Wang, W Gao, Y Fan, F Li, Z Zhou, C Gao, J Xiong, S Zhou, S Zhang, G Yang, R Jiang, J Zhang
    Brain research bulletin, 2021;172(0):108-119.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Changes in Gene and Protein Expression of Metalloproteinase-2 and -9 and Their Inhibitors TIMP2 and TIMP3 in Different Parts of Fluoride-Exposed Rat Brain
    Authors: A ?ukomska, I Baranowska, K Dec, A Pilutin, M Tarnowski, K Jakubczyk, W ?wiere??o, M Skórka-Maj, D Chlubek, I Gutowska
    International Journal of Molecular Sciences, 2020;22(1):.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. The antihypertensive agent hydralazine reduced extracellular matrix synthesis and liver fibrosis in nonalcoholic steatohepatitis exacerbated by hypertension
    Authors: Y Yuan, H Naito, K Kitamori, S Hashimoto, T Asano, T Nakajima
    PLoS ONE, 2020;15(12):e0243846.
    Species: Rat
    Sample Types: Serum
  4. Cardioprotective effect of MMP-2-inhibitor-NO-donor hybrid against ischaemia/reperfusion injury
    Authors: A Krzywonos-, A Franczak, A Olejnik, M Radomski, JF Gilmer, G Sawicki, M Wo?niak, I Bil-Lula
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  5. Liposomal Curcumin is Better than Curcumin to Alleviate Complications in Experimental Diabetic Mellitus
    Authors: AE Bulboac?, AS Porfire, LR Tefas, PM Boarescu, SD Bolboac?, IC St?nescu, AC Bulboac?, G Dogaru
    Molecules, 2019;24(5):.
    Species: Rat
    Sample Types: Plasma
  6. Matrix Metalloproteinase-Mediated Blood-Brain Barrier Dysfunction in Epilepsy
    Authors: RG Rempe, AMS Hartz, ELB Soldner, BS Sokola, SR Alluri, EL Abner, RJ Kryscio, A Pekcec, J Schlichtig, B Bauer
    J. Neurosci., 2018;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  7. 2-Methoxyestradiol Causes MMP 9 Mediated Transactivation of EGFR and Angiotensin Type 1 Receptor Downregulation in Rat Aortic Smooth Muscle Cells
    Authors: B Ogola, Y Zhang, L Iyer, T Thekkumkar
    Am. J. Physiol., Cell Physiol., 2018;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  8. Implication of Free Fatty Acids in Thrombin Generation and Fibrinolysis in Vascular Inflammation in Zucker Rats and Evolution with Aging
    Authors: J Lagrange, M Didelot, A Mohamadi, LA Walton, S Bloemen, B de Laat, H Louis, SN Thornton, B Derby, MJ Sherratt, B Fève, P Challande, R Akhtar, JK Cruickshan, P Lacolley, V Regnault
    Front Physiol, 2017;8(0):949.
    Species: Rat
    Sample Types: Plasma
  9. Ferulaldehyde Improves the Effect of Methotrexate in Experimental Arthritis
    Authors: L Slovák, K Švík, D Mihalová, J Tóth, S Czigle, ? Pašková, F Bilka, K Bauerová
    Molecules, 2017;22(11):.
    Species: Rat
    Sample Types: Plasma
  10. Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
    Authors: K Yan, Y Shen
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Serum
  11. Resveratrol suppresses neuroinflammation in the experimental paradigm of autism spectrum disorders
    Authors: Ranjana Bhandari
    Neurochem. Int, 2016;103(0):8-23.
    Species: Rat
    Sample Types: Serum

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Reviews for Rat Total MMP-9 Quantikine ELISA Kit

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Rat Total MMP-9 Quantikine ELISA Kit
By Anonymous on 11/06/2020
Sample Tested: Rat serum,Serum

I followed the recommended 10-fold dilution and the kit worked perfectly fine.

Rat Total MMP-9 Quantikine ELISA Kit
By Veena Rao on 06/09/2019
Sample Tested: EDTA Plasma
Rat Total MMP-9 Quantikine ELISA Kit RMP900
Rat Total MMP-9 Quantikine ELISA Kit
By BENARD OGOLA on 05/11/2017
Sample Tested: Human cell conditioned medium

Easy to follow and extrapolate graph for experiments