RB1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56888

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Equine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RB1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 251-268 (RTPRRGQNRSARIAKQLE) of human Rb was used as immunogen

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RB1 Antibody - BSA Free

Western Blot: RB1 Antibody [NB100-56888]

Western Blot: RB1 Antibody [NB100-56888]

Western Blot: RB1 Antibody [NB100-56888] - Analysis of Retinoblastoma 1 in 30 ugs of Jurkat cell lysate using NB100-56888 at 1:1000 dilution.

Applications for RB1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:200-1:1000
Application Notes
In Jurkat, a 110 kDa band is observed.

Reviewed Applications

Read 3 reviews rated 4 using NB100-56888 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RB1

Mutation or deregulation of Rb, retinoblastoma tumor suppressor gene, is seen so frequently in tumor cells that it is believe that some type of suppression of the Rb gene is required for cancer cell genesis.

Long Name

Retinoblastoma-associated protein

Alternate Names

OSRC, p105-Rb, pp110

Entrez Gene IDs

5925 (Human); 19645 (Mouse); 24708 (Rat)

Gene Symbol

RB1

UniProt

P06400

Additional RB1 Products

Product Documents for RB1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle

Customer Reviews for RB1 Antibody - BSA Free (3)

4 out of 5
3 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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2 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Detection of endogenous mouse RB1
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain (cortex) tissue
    Species: Mouse
    Verified Customer | Posted 02/03/2025
    Detection of endogenous RB1 in mouse cortex lysates.
    1:1000 dilution at 4 degrees overnight to detect endogenous RB1 from mouse (10 ug total protein).
    RB1 Antibody - BSA Free NB100-56888
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell extract form Jurkat cells
    Species: Human
    Verified Customer | Posted 07/31/2014
    WB for hsitone RB in Jurkat cells
    RB1 Antibody - BSA Free NB100-56888
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell extract from 293T cells
    Species: Human
    Verified Customer | Posted 07/25/2014
    RB protein in 293T cells
    RB1 Antibody - BSA Free NB100-56888

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