RBM3 Antibody (CL0296) - BSA Free

Novus Biologicals | Catalog # NBP2-52906

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0296

Format

BSA Free
View all RBM3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P98179, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RBM3 Antibody (CL0296) - BSA Free

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906] - Staining in human tonsil and liver tissues. Corresponding RBM3 RNA-seq data are presented for the same tissues.
Western Blot: RBM3 Antibody (CL0296) [NBP2-52906]

Western Blot: RBM3 Antibody (CL0296) [NBP2-52906]

Western Blot: RBM3 Antibody (CL0296) [NBP2-52906] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-RBM3 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.
Immunocytochemistry/ Immunofluorescence: RBM3 Antibody (CL0296) [NBP2-52906]

Immunocytochemistry/ Immunofluorescence: RBM3 Antibody (CL0296) [NBP2-52906]

Immunocytochemistry/Immunofluorescence: RBM3 Antibody (CL0296) [NBP2-52906] - Staining of U-2 OS cells using the anti-RBM3 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Western Blot: RBM3 Antibody (CL0296) [NBP2-52906]

Western Blot: RBM3 Antibody (CL0296) [NBP2-52906]

Western Blot: RBM3 Antibody (CL0296) [NBP2-52906] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906] - Staining of human tonsil shows moderate nuclear positivity in lymphoid cells, mainly in germinal centra.
Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906] - Staining of human rectum shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906] - Staining of human breast cancer shows moderate nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906] - Staining of human colorectal cancer shows weak to moderate nuclear positivity in a subset of tumor cells.
Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906]

Immunohistochemistry-Paraffin: RBM3 Antibody (CL0296) [NBP2-52906] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for RBM3 Antibody (CL0296) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBM3

RBM3 is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq]

Alternate Names

IS1-RNPL, putative RNA-binding protein 3, RNA binding motif (RNP1, RRM) protein 3, RNA binding motif protein 3, RNA-binding motif protein 3, RNPL

Gene Symbol

RBM3

Additional RBM3 Products

Product Documents for RBM3 Antibody (CL0296) - BSA Free

Certificate of Analysis

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Product Specific Notices for RBM3 Antibody (CL0296) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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