RC74 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-46468

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RC74 Primary Antibodies »

Product Specifications

Immunogen

integrator complex subunit 9. (Uniprot# Q9NV88)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RC74 Antibody - BSA Free

RC74 Antibody - BSA Free

Western Blot: RC74 Antibody - BSA Free [NBP3-46468] -

Western Blot: RC74 Antibody - BSA Free [NBP3-46468] - Jurkat cells were subjected to SDS PAGE followed by western blot with (RC74 antibody) at dilution of 1:1000
RC74 Antibody - BSA Free

Immunoprecipitation: RC74 Antibody - BSA Free [NBP3-46468] -

Immunoprecipitation: RC74 Antibody - BSA Free [NBP3-46468] - IP Result of anti-RC74 (IP:, 4ug; Detection: 1:1000) with HeLa cells lysate 880ug.

Applications for RC74 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:200-1:2000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RC74

Formation of the mature 3' ends of the vast majority of cellular mRNAs occurs through cleavage and polyadenylation and requires a cleavage and polyadenylation specificity factor (CPSF) containing, among other proteins, CPSF-73 and CPSF-100. RC-74 is an exclusively nuclear protein found in HeLa cells that has similar characteristics to CPSF-100. Like the complex that forms between CPSF-100 and CPSF-73, studies show that RC-74 may form a similar complex with RC-68 that is independent from the CPSF complex. This RC-68 / RC-74 complex may be involved in a pre-mRNA processing event other than cleavage and polyadenylation, such as the 3' end processing of a distinct subset of cellular pre-mRNAs encoding proteins required for G(1) progression and entry into S phase.

Alternate Names

CPSF2L, FLJ10871, Int9, integrator complex subunit 9, Protein related to CPSF subunits of 74 kDa, RC74, RC-74

Gene Symbol

INTS9

Additional RC74 Products

Product Documents for RC74 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RC74 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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