RCC2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35311

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RCC2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 323-522 of human RCC2 (NP_001129676.1).

Sequence:
QILPVPNVVVRDVACGANHTLVLDSQKRVFSWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGASQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMYPKAVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVAMGYSHSLVIARDESETEKEKIKKLPEYNPRTL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RCC2 Antibody - BSA Free

RCC2 Antibody

Western Blot: RCC2 Antibody [NBP3-35311] -

Western Blot: RCC2 Antibody [NBP3-35311] - Western blot analysis of various lysates using RCC2 Rabbit pAb at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for RCC2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RCC2

RCC2 (regulator of chromosome condensation 2) is a member of the RCC1 family of guanine nucleotide exchange factors. RCC2 has been identified as part of the chromosomal passenger complex (CPC) which coordinates the chromosomal and cytoskeletal events of mitosis. Chromosomal passengers are proteins present in complexes with aurora B, inner centromeric protein (INCENP), survivin, and borealin. RCC2 has been demonstrated to be required for the recruitment of survivin and aurora B and is essential for proper chromosome segregation and the completion of cell cleavage. Loss of RCC2 inhibits spindle assembly, activates the spindle assembly checkpoint, and blocks cells in prometaphase.

Alternate Names

DKFZp762N0610, KIAA1470, protein RCC2, RCC1-like protein TD-60, regulator of chromosome condensation 2, TD60, TD-60, Telophase disk protein of 60 kDa

Gene Symbol

RCC2

Additional RCC2 Products

Product Documents for RCC2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RCC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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