RCN2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32010

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RCN2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RCN2 Antibody - BSA Free

Western Blot: RCN2 Antibody [NBP2-32010]

Western Blot: RCN2 Antibody [NBP2-32010]

Western Blot: RCN2 Antibody [NBP2-32010] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: RCN2 Antibody [NBP2-32010]

Immunocytochemistry/ Immunofluorescence: RCN2 Antibody [NBP2-32010]

Immunocytochemistry/Immunofluorescence: RCN2 Antibody [NBP2-32010] - Staining of human cell line U-251 MG shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010] - Staining of human cerebral cortex.
Immunohistochemistry: RCN2 Antibody [NBP2-32010]

Immunohistochemistry: RCN2 Antibody [NBP2-32010]

Immunohistochemistry: RCN2 Antibody [NBP2-32010] - Staining of mouse locus coeruleus shows immunoreactivity in neurons.
Immunohistochemistry: RCN2 Antibody [NBP2-32010]

Immunohistochemistry: RCN2 Antibody [NBP2-32010]

Immunohistochemistry: RCN2 Antibody [NBP2-32010] - Staining of mouse red nucleus shows strong immunoreactivity in neurons.
Immunohistochemistry: RCN2 Antibody [NBP2-32010]

Immunohistochemistry: RCN2 Antibody [NBP2-32010]

Immunohistochemistry: RCN2 Antibody [NBP2-32010] - Staining of mouse thalamus shows positivity in neurons of the parafascicular nucleus.
Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010] - Staining of human cerebral cortex, epididymis, pancreas and testis using Anti-RCN2 antibody NBP2-32010 (A) shows similar protein distribution across tissues to independent antibody NBP2-32011 (B).
Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010] - Staining of human pancreas.
Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010] - Staining of human testis.
Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010]

Immunohistochemistry-Paraffin: RCN2 Antibody [NBP2-32010] - Staining of human epididymis.

Applications for RCN2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RCN2

Reticulocalbin 2 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. The RCN2 gene maps to the same region as type 4 Bardet-Biedl syndrome (MIM:600374), suggesting a possible causative role for reticulocalbin 2 in the disorder. [provided by RefSeq]

Alternate Names

Calcium-binding protein ERC-55, E6BPreticulocalbin 2, EF-hand calcium binding domain (endoplasmic reticulumcalcium-binding protein, 55kD), ERC-55, ERC55E6-binding protein, reticulocalbin 2, EF-hand calcium binding domain, reticulocalbin-2, TCBP49

Gene Symbol

RCN2

UniProt

Q14257 (Human)

Additional RCN2 Products

Product Documents for RCN2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RCN2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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