Recombinant Canine Erythropoietin/EPO Protein

Carrier Free

Catalog # Availability Size / Price Qty
3816-CE-010/CF

With Carrier

Catalog # Availability Size / Price Qty
3816-CE-010

15% Off Limited-time Savings on Your Reagents, Assays, and Kits
Now through September 27th, you can save 15% on Bio-Techne reagents, assays, and kits (including Novus, Tocris, and R&D Systems). Use promotion code SAVE15 at checkout on orders over $1,000. See terms and conditions for offer details.

R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Reviews (2)

Recombinant Canine Erythropoietin/EPO Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.4-2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived canine Erythropoietin/EPO protein
Ala41-Arg206
Accession #
N-terminal Sequence
Analysis
Ala41
Predicted Molecular Mass
18.4 kDa
SDS-PAGE
36-38 kDa, reducing conditions

Product Datasheets

You must select a language.

x

3816-CE (with carrier)

You must select a language.

x

3816-CE/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3816-CE

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3816-CE/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Erythropoietin/EPO

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2). Glycosylation of EPO is required for biological activities in vivo (3). Mature canine EPO shares 95% amino acid sequence identity with feline EPO and 80%-89% with bovine, equine, human, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (4). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (5-7). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R) (7, 8). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (6, 7, 9, 10). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 11). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 5). EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (6, 7, 12).

References
  1. Koury, M.J. (2005) Exp. Hematol. 33:1263.
  2. Wen, D. et al. (1993) Blood 82:1507.
  3. Tsuda E., et al. (1990) Eur. J. Biochem. 188:405.
  4. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  5. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  6. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  7. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  8. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  9. Acs, G. et al. (2001) Cancer Res. 61:3561.
  10. Hardee, M.E. et al. (2006)Clin. Cancer Res. 12:332.
  11. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  12. Kertesz, N. et al. (2004) Dev. Biol. 276:101.
Entrez Gene IDs
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Canine Erythropoietin/EPO Protein

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Recombinant Canine Erythropoietin/EPO Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Recombinant Canine Erythropoietin Protein
By Anonymous on 12/06/2017
Application: In vitro bioactivity in cell culture

Recombinant Canine Erythropoietin Protein
By Anonymous on 01/13/2016
Application: In vitro bioactivity in cell culture