>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey 4-1BB/TNFRSF9/CD137 Fc Chimera is immobilized at 0.05 µg/mL (100 µL/well),
Human 4-1BB Ligand/TNFSF9 (Catalog # 2295-4L)
binds with an ED50 of 0.25-1.25 ng/mL.
When Recombinant Cynomolgus Monkey 4‑1BB/TNFRSF9Fc Chimera is coated at 0.05 μg/mL, 100 μL/well, Recombinant Human 4-1BB Ligand/TNFSF9 (Catalog # 2295-4L) binds with an ED50 of 0.25-1.25 ng/mL.
also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane
glycoprotein in the TNF receptor superfamily. 4-1BB functions in the
development and activation of multiple immune cells (1). Mature human 4-1BB
consists of a 163 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine‑rich
repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain (2, 3).
Within the ECD, cynomolgus 4-1BB shares 95%, 57%, and 57% aa sequence identity
with human, mouse, and rat 4-1BB, respectively.
4-1BB is expressed as a
disulfide-linked homodimer on various populations of activated T cell including
CD4+, CD8+, memory CD8+, NKT, and regulatory T cells (4-7) as well as on
myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially
infected osteoblasts (8-11). It binds with high affinity to the transmembrane
4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid
progenitor cells (3, 8). This interaction co-stimulates the proliferation, activation, and/or
survival of the 4-1BB expressing cell (3-7). It can also enhance the activation-induced
cell death of repetitively stimulated T cells (3). Mice lacking 4-1BB show
augmented T cell activation, perhaps due to its absence on regulatory T cells
(12). 4-1BB can associate with OX40 on activated T cells, forming a complex
that responds to either ligand and inhibits Treg and CD8+ T cell proliferation
(13). Reverse signaling through 4-1BB Ligand inhibits the development of
dendritic cells, B cells, and osteoclasts (8, 11) but supports mature dendritic
cell survival and co‑stimulates the proliferation and activation of
mast cells (9, 10). 4-1BB activation enhances CD8+ T cell and NK cell
mediated anti-tumor immunity (14). It also contributes to the development of
inflammation in high fat diet-induced metabolic syndrome (15). Soluble forms of
4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid
arthritis and hematologic cancer patients, respectively (16, 17).
Wang, C. et al. (2009) Immunol. Rev. 229:192.
Schwarz, H. et al. (1993) Gene 134:295.
Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
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