Recombinant Cynomolgus B7-H7/HHLA2 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10109-B7-050
Recombinant Cynomolgus B7-H7/HHLA2 His-tag Protein, CF Binding Activity
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Recombinant Cynomolgus B7-H7/HHLA2 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Human TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Cynomolgus Monkey B7-H7/HHLA2 (Catalog#10109-B7)that produces 50% of the optimal binding response is 0.2-1.2 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey B7-H7/HHLA2 protein
Ile21-Asn345, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ile21
Predicted Molecular Mass
38 kDa
SDS-PAGE
62-70 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10109-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR) is coated at 2 μg/mL (100 μL/well), Recombinant Cynomolgus B7-H7/HHLA2 (Catalog # 10109-B7) binds with an ED50 of 0.2‑1.2 μg/mL

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey B7-H7/HHLA2 (Catalog#10109-B7) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 62-70 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H7/HHLA2

B7-H7, also known as HHLA2 (HERV-H LTR-associating protein 2), is a member of the B7 family of immune regulatory proteins (1, 2). The mature human B7-H7 protein consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). Within the ECD, cynomolgus B7-H7 shares 90% aa sequence identity with human B7-H7. It is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). The receptor TMIGD2, commonly found in endothelial and epithelial cells, has also been identified as one of the receptors for B7-H7 (6). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma, TNF-alpha, IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

References
  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.
  6. Janakiram, M. et al. (2015) Oncoimmunology 4:8.
Long Name
B7 Homolog 7
Entrez Gene IDs
11148 (Human); 102137704 (Cynomolgus Monkey)
Alternate Names
B7H7; B7-H7; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; HHLA2; Human endogenous retrovirus-H long terminal repeat-associating protein 2

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