Recombinant Cynomolgus CD155/PVR Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10058-CD-050
Recombinant Cynomolgus CD155/PVR Fc Chimera Protein, CF Binding Activity
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Recombinant Cynomolgus CD155/PVR Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CD155/PVR Fc Chimera is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human CD96v2 Fc Chimera  (Catalog # 9556-CD) that produces 50% optimal binding response is 0.25-1.25 μg/mL.   
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey CD155/PVR protein
Cynomolgus Monkey CD155/PVR
(Asp28-Asn343)
Accession # XP_011734201.1 (T77M, V93T, G223S, R328G)
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Asp28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85 kDa
SDS-PAGE
68-77 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10058-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Cynomolgus Monkey CD155/PVR Fc Chimera (Catalog # 10058-CD) is coated at 5 μg/mL, 100 μL/well, Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) binds with an ED50 of 0.25-1.25 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey CD155/PVR Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 68-77 kDa and 140-150 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155 may play a role in cancer cell invasion and migration, and binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homophilicly (3, 4). The mature human CD155 consists of a 323 amino acid (aa) extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa transmembrane segment, and a 50 aa cytoplasmic tail. The sequence of the cynomolgus CD155/PVR was isolated from a cynomolgus monkey cDNA library and aligns with the sequence for Macaca nemestrina (XP_011734201.1 aa24-339, T77M, V93T, G223S, R328G). CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 5-8). It is preferentially expressed on Th17 cells compared to Th2 cells (9), and its activation promotes the development of Th1 responses (10). On migrating cells, CD155 is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 11). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM‑1 or CD96 expressing NK cells (1, 6, 12). Binding of monocyte DNAM‑1 to endothelial cell CD155 promotes transendothelial migration (7).

References
  1. Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197.
  2. Ravens, I. et al. (2003) Biochem. Biophys. Res. Commun. 312:1364.
  3. Sloan, K. et al. (2004) BMC Cancer. 4:73.
  4. Sato, T. et al. (2004) Genes to Cells 9:791.
  5. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177.
  6. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Maier, M.K. et al. (2007) Eur. J. Immunol. 37:2214.
  9. Lozano, E. et al. (2013) J. Immunol. 191:3673.
  10. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644.
  11. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
  12. Chan, C.J. et al. (2010) J. Immunol. 184:902.
Long Name
Poliovirus Receptor
Entrez Gene IDs
5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)
Alternate Names
CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4

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