Recombinant Cynomolgus Fc gamma RIII/CD16 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Fc gamma RIII /CD16 was immobilize on a His Tag antibody coated plate, it binds biotinylated Human IgG. The concentration of biotinylated Human IgG that produces 50% of the optimal binding response is approximately 0.15-0.75 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Gly17-Gln208, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly17, Glu21 and Met18
  • Predicted Molecular Mass
    23 kDa
  • SDS-PAGE
    36-44 kDa, reducing conditions
9224-FC
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Data Images
Binding Activity
When Recombinant Cynomolgus Fc gamma RIII /CD16 (Catalog #
9224-FC) was immobilized on a His Tag antibody (Catalog # MAB050) coated plate, it binds Biotinylated Human IgG with an ED50 of 0.15-0.75 μg/mL.
Background: Fc gamma RIII (CD16)
Fc gamma  RIII/CD16 is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes (1-3). Mature cynomolgus Fc gamma  RIII consists of a 192 aa ECD with two C2-type
Ig-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain (4). In humans, Fc gamma  RIIIA/CD16a is expressed as a 50-70 kDa transmembrane activating receptor on NK cells, T cells, monocytes, and macrophages (2). It is closely related to the GPI-linked Fc gamma RIIIB which is expressed on human neutrophils and eosinophils (1, 3). These two proteins share 97% amino acid (aa) identity within their extracellular domains (ECD) (5). Within the ECD, mature cynomolgus Fc gamma RIII shares 92% and 90% aa sequence identity with human Fc gamma  RIIIA and Fc gamma  RIIIB, respectively. Fc gamma RIII surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta (8, 9). Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA (10). Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity (11-14). Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis (14). Soluble Fc gamma  RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases (12, 13). Cynomolgus Fc gamma RIII binds to cynomolgus IgG subclasses 1-4, to human IgG1 and 3, and more weakly to human IgG2 and 4 (15).
  • References:
    1. Nagelkerke, S.Q. and T.W. Kuijpers (2015) Front. Immunol. 5:674.
    2. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
    3. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
    4. Rogers, K.A. et al. (2008) J. Immunol. 177:3848.
    5. Scallon, B.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5079.
    6. Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
    7. Dall'Ozzo, S. et al. (2004) Cancer Res. 64:4664.
    8. Kim, M.-K. et al. (2003) Blood 101:4479.
    9. Lanier, L.L. et al. (1989) Nature 342:803.
    10. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.    
    11. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
    12. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
    13. Masuda, M. et al. (2006) Atherosclerosis 188:377.
    14. Webster, N.L. et al. (2006) J. Leukoc. Biol. 79:294.
    15. Warncke, M. et al. (2012) J. Immunol. 88:4405.
  • Long Name:
    Fc gamma Receptor III
  • Entrez Gene IDs:
    14131 (Mouse); 102140945 (Cynomolgus Monkey)
  • Alternate Names:
    Fc gamma RIII (CD16); FcgRIII
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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