Recombinant Cynomolgus HVEM/TNFRSF14 Fc Chimera Protein

  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey HVEM/TNFRSF14 Fc Chimera is coated at 2 µg/mL (100 μL/well), biotinylated recombinant mouse BTLA Fc Chimera binds with a typical ED50 of 150-750 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Cynomololgus Monkey HVEM/TNFRSF14
    Accession # XP_005545061
    IEGRMD Human IgG1
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    44 kDa
    56-63 kDa, reducing conditions
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
WhenRecombinant Cymomologus HVEM/TNFRSF14 Fc Chimera (Catalog # 9197-HV) is coated at2 μg/mL, Recombinant  Biotinylated Mouse BTLA  Fc Chimera binds with a typicalED50 of 150-750 ng/mL.
Background: HVEM/TNFRSF14
HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). Mature cynomolgous HVEM consists of a 171 amino acid (aa) extracellular domain (ECD) with three cysteine-rich domains (CRD), a 24 aa transmembrane segment, and a 42 aa cytoplasmic tail with a TRAF interaction domain (2, 3). Within the ECD, cynomolgous HVEM shares 88%, 54%, and 54% aa sequence identity with human, mouse, and rat HVEM, respectively. HVEM is highly expressed on naïve CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils (4-8). Its expression declines during effector T cell activation but is up-regulated during Treg activation (4, 5). HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-a (4, 9-12). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (8, 10) and contributes to Th1 inflammation and cardiac allograft rejection (13, 14). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (4, 7, 9, 10) and dampens intestinal inflammation (15). HVEM enhances the development of CD8+ T cell memory and Treg function (5, 6). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense (16). The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (2, 9, 11).
  • References:
    1. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
    2. Montgomery, R.I. et al. (1996) Cell 87:427.
    3. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
    4. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
    5. Tao, R. et al. (2008) J. Immunol. 180:6649.
    6. Steinberg, M.W. et al. (2013) PLoS One 8:e77992.
    7. de Trez, C. et al. (2008) J. Immunol. 180:238.
    8. Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330.
    9. Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
    10. Cai, G. et al. (2008) Nat. Immunol. 9:176.
    11. Mauri, D.N. et al. (1998) Immunity 8:21.
    12. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
    13. Wang, J. et al. (2005) J. Immunol. 174:8173.
    14. Ye, Q. et al. (2002) J. Exp. Med. 195:795.
    15. Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463.
    16. Shui, J.W. et al. (2012) Nature 488:222.
  • Long Name:
    Herpesvirus Entry Mediator
  • Entrez Gene IDs:
    8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
  • Alternate Names:
    ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2
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