Recombinant Cynomolgus ICOS Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9736-CS-100
Recombinant Cynomolgus ICOS Fc Chimera Protein Bioactivity
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Recombinant Cynomolgus ICOS Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-H2 (Catalog # 9327-B7) is coated at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey ICOS Fc Chimera that produces 50% optimal binding response is 0.75-3.75 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey ICOS protein
Cynomolgous Monkey ICOS
(Glu21-Gln134)
Accession # XP_005574075
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Glu21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa
SDS-PAGE
40-61 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9736-CS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Cynomolgus ICOS Fc Chimera Protein Bioactivity View Larger

Recombinant Cynomolgus Monkey B7-H2 (Catalog # 9327-B7) is immobilized at 0.5 µg/mL, 100 µL/well, Recombinant Cynomolgus Monkey ICOS Fc Chimera (Catalog # 9736-CS) binds with an ED50 of 0.75-3.75 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICOS

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune co-stimulatory receptors (1, 5). Other family members are CD28, CTLA-4 and PD-1 (2, 4). ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Cynomolgus monkey ICOS is a homodimeric type I transmembrane protein consisting of a putative signal sequence, an extracellular domain, a transmembrane region, and a cytoplasmic domain. Over aa 21-134, cynomolgus monkey ICOS shares 99% and 70% aa sequence identity with human and mouse ICOS, respectively. ICOS is expressed on most CD45RO+ cells (3). ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells (4, 6). B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand (4, 5). The B7‑H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation (5, 7).

References
  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Khayyamian, S. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99:6198.
  4. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  5. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  6. Hutloff, A. et al. (1999) Nature 397:263.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
Inducible Co-stimulator
Entrez Gene IDs
29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names
Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850

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