Recombinant Cynomolgus IL-10 R alpha Fc Chimera Protein, CF Summary
|Cynomolgus Monkey IL-10 R alpha|
Accession # XP_005579838.1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Cynomolgus Monkey IL‑10 R alpha Fc Chimera (Catalog # 10186-RI) inhibits IL-10-dependent proliferation of MC/9‑2 mouse mast cells. The ED50 for this effect is 2-12 ng/mL.
2 μg/lane of Recombinant Cynomolgus Monkey IL-10 R alpha Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 70-80 kDa and 140-160 kDa, respectively.
Background: IL-10 R alpha
Interleukin-10 Receptor alpha (IL-10 R alpha), also known as IL-10 R1, is a 51 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2-5). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL‑26, IL-28, and IL-29 (1). Polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature IL-10 R alpha consists of a extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain (12). Within the ECD, cynomolgus monkey IL-10 R alpha shares 95%, 59% and 60% amino acid identity with Human, mouse, and rat IL-10 R alpha, respectively.
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