Recombinant Cynomolgus IL-10 R alpha Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10186-RI-050
Recombinant Cynomolgus IL-10 R alpha Fc Chimera Protein, CF Inhibition Activity
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Recombinant Cynomolgus IL-10 R alpha Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-10-dependent proliferation of MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 2-12 ng/mL in the presence of 2 ng/mL Recombinant Human IL‑10 (Catalog # 217-IL).
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-10 R alpha protein
Cynomolgus Monkey IL-10 R alpha
(His22-Asn235)
Accession # XP_005579838.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
His22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa
SDS-PAGE
70-80 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10186-RI

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Inhibition Activity Inhibition Activity View Larger

Recombinant Cynomolgus Monkey IL‑10 R alpha Fc Chimera (Catalog # 10186-RI) inhibits IL-10-dependent proliferationof MC/9‑2mouse mast cells. The ED50 for this effect is 2-12 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey IL-10 R alpha Fc Chimera was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® blue staining, showing bands at 70-80 kDa and 140-160 kDa,respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10 R alpha

Interleukin-10 Receptor alpha (IL-10 R alpha), also known as IL-10 R1, is a 51 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2-5). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL‑26, IL-28, and IL-29 (1). Polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature IL-10 R alpha consists of a extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain (12). Within the ECD, cynomolgus monkey IL-10 R alpha shares 95%, 59% and 60% amino acid identity with Human, mouse, and rat IL-10 R alpha, respectively.

References
  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
  3. Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
  4. Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
  5. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  6. Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053.
  7. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  8. Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906.
  9. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
  10. Fernandez, S. et al. (2004) J. Immunol. 172:2613.
  11. Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365.
  12. Liu, Y. et al. (1994) J. Immunol. 152:1821.
Long Name
Interleukin 10 Receptor alpha
Entrez Gene IDs
3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)
Alternate Names
CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha

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