Recombinant Human IL-10 Protein

Formulations:
Catalog # Availability Size / Price Qty
217-IL-005
217-IL-025
217-IL-250
217-IL-050
217-IL-010
217-IL-01M
Product Details
Citations (86)
FAQs
Reviews (5)

Recombinant Human IL-10 Protein Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.15-0.75 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-10 protein
Ser19-Asn178
Accession #
N-terminal Sequence
Analysis
Ser19
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
18.6 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

217-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

217-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Reconstitute at 50 µg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha ‑helical cytokines that also includes
IL‑19, IL‑20, IL‑22, IL‑24, and IL‑26/AK155 (1, 2). IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature human IL‑10 shares 72%‑86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL‑10 is active on mouse cells, mouse IL‑10 does not act on human cells (6, 7). IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL‑10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta /IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL‑10 R beta does not bind IL‑10 by itself but is required for signal transduction (1). IL‑10 R beta also associates with IL‑20 R alpha, IL‑22R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29
(11‑13). IL‑10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14‑16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

References
  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. Sabat, R. et al. (2010) Cytokine Growth Factor Rev. 21:331.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  9. Syto, R. et al. (1998) Biochemistry 37:16943.
  10. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  11. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  12. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  13. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  14. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  15. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  16. Blackburn, S.D. and E.J. Wherry (2007)Trends Microbiol. 15:143.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Citations for Recombinant Human IL-10 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

86 Citations: Showing 1 - 10
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  1. Large-scale reduction of tyrosine kinase activities in human monocytes stimulated in vitro with N. meningitidis
    Authors: U Gopinathan, KR Redalen, AM Trøseid, P Kierulf, P Brandtzaeg, AH Ree, JP Berg, R Øvstebø
    PLoS ONE, 2018;13(1):e0181912.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The PI3K?-Selective Inhibitor Idelalisib Minimally Interferes with Immune Effector Function Mediated by Rituximab or Obinutuzumab and Significantly Augments B Cell Depletion In Vivo
    Authors: A Palazzo, S Herter, L Grosmaire, R Jones, CR Frey, F Limani, M Bacac, P Umana, RJ Oldham, MJE Marshall, KL Cox, AH Turaj, MS Cragg, C Klein, MJ Carter, S Tannheimer
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Vitamin D status contributes to the antimicrobial activity of macrophages against Mycobacterium leprae
    Authors: EW Kim, RMB Teles, S Haile, PT Liu, RL Modlin
    PLoS Negl Trop Dis, 2018;12(7):e0006608.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Salmonella Activation of STAT3 Signaling by SarA Effector Promotes Intracellular Replication and Production of IL-10
    Authors: SL Jaslow, KD Gibbs, WF Fricke, L Wang, KJ Pittman, MK Mammel, JT Thaden, VG Fowler, GE Hammer, JR Elfenbein, DC Ko
    Cell Rep, 2018;23(12):3525-3536.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. CD46 activation induces distinct CXCL-10 response in monocytes and monocyte-derived dendritic cells
    Authors: AS Hansen, M Biltoft, B Bundgaard, AB Bohn, BK Møller, P Höllsberg
    Cytokine, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Interleukin 2 and interleukin 10 function synergistically to promote CD8(+) T cell cytotoxicity, which is suppressed by regulatory T cells in breast cancer
    Authors: X Li, P Lu, B Li, W Zhang, R Yang, Y Chu, K Luo
    Int. J. Biochem. Cell Biol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
    Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
    PLoS ONE, 2017;12(10):e0186317.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Lipid profiling of polarized human monocyte-derived macrophages
    Authors: David M Aronoff
    Prostaglandins Other Lipid Mediat., 2016;127(0):1-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Environmental factor and inflammation-driven alteration of the total peripheral T-cell compartment in granulomatosis with polyangiitis
    Authors: Anja Kerstein
    J. Autoimmun, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Tyrosine kinase 2 is not limiting human antiviral type III interferon responses
    Eur J Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
    Sci Rep, 2016;6(0):36795.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Imprime PGG-Mediated Anti-Cancer Immune Activation Requires Immune Complex Formation
    PLoS ONE, 2016;11(11):e0165909.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Macrophages Enhance Migration in Inflammatory Breast Cancer Cells via RhoC GTPase Signaling
    Sci Rep, 2016;6(0):39190.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Novel mechanism of macrophage-mediated metastasis revealed in a zebrafish model of tumor development.
    Authors: Wang J, Cao Z, Zhang X, Nakamura M, Sun M, Hartman J, Harris R, Sun Y, Cao Y
    Cancer Res, 2015;75(2):306-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Leishmania infantum amastigotes trigger a subpopulation of human B cells with an immunoregulatory phenotype.
    Authors: Andreani G, Ouellet M, Menasria R, Gomez A, Barat C, Tremblay M
    PLoS Negl Trop Dis, 2015;9(2):e0003543.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Human umbilical cord blood mesenchymal stem cell-derived PGE2 and TGF-beta1 alleviate atopic dermatitis by reducing mast cell degranulation.
    Authors: Kim H, Yun J, Shin T, Lee S, Lee B, Yu K, Seo Y, Lee S, Kang T, Choi S, Seo K, Kang K
    Stem Cells, 2015;33(4):1254-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. MafB promotes atherosclerosis by inhibiting foam-cell apoptosis.
    Authors: Hamada, Michito, Nakamura, Megumi, Tran, Mai Thi, Moriguchi, Takashi, Hong, Cynthia, Ohsumi, Takayuki, Dinh, Tra Thi, Kusakabe, Manabu, Hattori, Motochik, Katsumata, Tokio, Arai, Satoko, Nakashima, Katsuhik, Kudo, Takashi, Kuroda, Etsushi, Wu, Chien-Hu, Kao, Pei-Han, Sakai, Masaharu, Shimano, Hitoshi, Miyazaki, Toru, Tontonoz, Peter, Takahashi, Satoru
    Nat Commun, 2014;5(0):3147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells.
    Authors: Valle Oseguera, Cendy A, Spencer, Juliet V
    PLoS ONE, 2014;9(2):e88708.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Multiplexed screening of natural humoral immunity identifies antibodies at fine specificity for complex and dynamic viral targets.
    Authors: McCutcheon, Krista M, Gray, Julia, Chen, Natalie, Liu, Keyi, Park, Minha, Ellsworth, Stote, Tripp, Ralph A, Tompkins, S Mark, Johnson, Scott K, Samet, Shelly, Pereira, Lenore, Kauvar, Lawrence
    MAbs, 2014;6(2):460-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Cluster of differentiation 45 activation is crucial in interleukin-10-dependent tumor-associated dendritic cell differentiation.
    Authors: Cheng, DA-En, Tsai, Ying-Min, Hsu, Ya-Ling, Hou, Ming-Fen, Tsai, Eing-Mei, Wang, Jaw-Yuan, Kan, Jung-Yu, Kuo, Po-Lin
    Oncol Lett, 2014;8(2):620-626.
  21. Cytokines alter IgA1 O-glycosylation by dysregulating C1GalT1 and ST6GalNAc-II enzymes.
    Authors: Suzuki, Hitoshi, Raska, Milan, Yamada, Koshi, Moldoveanu, Zina, Julian, Bruce A, Wyatt, Robert J, Tomino, Yasuhiko, Gharavi, Ali G, Novak, Jan
    J Biol Chem, 2014;289(8):5330-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Tie2 signaling cooperates with TNF to promote the pro-inflammatory activation of human macrophages independently of macrophage functional phenotype.
    Authors: Garcia S, Krausz S, Ambarus C, Fernandez B, Hartkamp L, van Es I, Hamann J, Baeten D, Tak P, Reedquist K
    PLoS ONE, 2014;9(1):e82088.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Expansion of parasite-specific CD4+ and CD8+ T cells expressing IL-10 superfamily cytokine members and their regulation in human lymphatic filariasis.
    Authors: Anuradha R, George P, Hanna L, Kumaran P, Chandrasekaran V, Nutman T, Babu S
    PLoS Negl Trop Dis, 2014;8(4):e2762.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  24. Growth factor and Th2 cytokine signaling pathways converge at STAT6 to promote arginase expression in progressive experimental visceral leishmaniasis.
    Authors: Osorio E, Travi B, da Cruz A, Saldarriaga O, Medina A, Melby P
    PLoS Pathog, 2014;10(6):e1004165.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Polyclonal B cell activation for accurate analysis of pre-existing antigen-specific memory B cells.
    Authors: Karahan G, Eikmans M, Anholts J, Claas F, Heidt S
    Clin Exp Immunol, 2014;177(1):333-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Motor, visual and emotional deficits in mice after closed-head mild traumatic brain injury are alleviated by the novel CB2 inverse agonist SMM-189.
    Authors: Reiner A, Heldt S, Presley C, Guley N, Elberger A, Deng Y, D'Surney L, Rogers J, Ferrell J, Bu W, Del Mar N, Honig M, Gurley S, Moore B
    Int J Mol Sci, 2014;16(1):758-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Interleukin-6 (IL-6) trans signaling drives a STAT3-dependent pathway that leads to hyperactive transforming growth factor-beta (TGF-beta) signaling promoting SMAD3 activation and fibrosis via Gremlin protein.
    Authors: O'Reilly S, Ciechomska M, Cant R, van Laar J
    J Biol Chem, 2014;289(14):9952-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis.
    Authors: Johnston A, Xing X, Swindell W, Kochkodan J, Riblett M, Nair R, Stuart P, Ding J, Voorhees J, Elder J, Gudjonsson J
    Hum Mol Genet, 2013;22(9):1807-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. IL-17 mediates immunopathology in the absence of IL-10 following Leishmania major infection.
    Authors: Gonzalez-Lombana C, Gimblet C, Bacellar O, Oliveira W, Passos S, Carvalho L, Goldschmidt M, Carvalho E, Scott P
    PLoS Pathog, 2013;9(3):e1003243.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. TACI mutations and impaired B-cell function in subjects with CVID and healthy heterozygotes.
    Authors: Martinez-Gallo M, Radigan L, Almejun M, Martinez-Pomar N, Matamoros N, Cunningham-Rundles C
    J Allergy Clin Immunol, 2013;131(2):468-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
    Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
    J Allergy Clin Immunol, 2013;131(4):1146-56, 1156.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Unraveling the signaling pathways promoting fibrosis in Dupuytren's disease reveals TNF as a therapeutic target.
    Authors: Verjee L, Verhoekx J, Chan J, Krausgruber T, Nicolaidou V, Izadi D, Davidson D, Feldmann M, Midwood K, Nanchahal J
    Proc Natl Acad Sci U S A, 2013;110(10):E928-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Antigen-independent induction of Tim-3 expression on human T cells by the common gamma-chain cytokines IL-2, IL-7, IL-15, and IL-21 is associated with proliferation and is dependent on the phosphoinositide 3-kinase pathway.
    Authors: Mujib S, Jones RB, Lo C, Aidarus N, Clayton K, Sakhdari A, Benko E, Kovacs C, Ostrowski MA
    J. Immunol., 2012;188(8):3745-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Role of Smad proteins in resistance to BMP-induced growth inhibition in B-cell lymphoma.
    Authors: Huse K, Bakkebo M, Walchli S, Oksvold M, Hilden V, Forfang L, Bredahl M, Liestol K, Alizadeh A, Smeland E, Myklebust J
    PLoS ONE, 2012;7(10):e46117.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. The C-type Lectin Receptor CLECSF8 (CLEC4D) Is Expressed by Myeloid Cells and Triggers Cellular Activation through Syk Kinase.
    Authors: Graham LM, Gupta V, Schafer G, Reid DM, Kimberg M, Dennehy KM, Hornsell WG, Guler R, Campanero-Rhodes MA, Palma AS, Feizi T, Kim SK, Sobieszczuk P, Willment JA, Brown GD
    J. Biol. Chem., 2012;287(31):25964-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. IFN-alpha blocks IL-17 production by peripheral blood mononuclear cells in Behcet's disease.
    Authors: Liu X, Yang P, Wang C, Li F, Kijlstra A
    Rheumatology (Oxford), 2011;50(2):293-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Toll-like receptor 9 signaling by CpG-B oligodeoxynucleotides induces an apoptotic pathway in human chronic lymphocytic leukemia B cells.
    Authors: Liang X, Moseman EA, Farrar MA
    Blood, 2010;115(24):5041-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. IL-16 promotes T. whipplei replication by inhibiting phagosome conversion and modulating macrophage activation.
    Authors: Ghigo E, Barry AO, Pretat L, Al Moussawi K, Desnues B, Capo C, Kornfeld H, Mege JL
    PLoS ONE, 2010;5(10):e13561.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010;185(11):7047-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. PEGylation of interleukin-10 for the mitigation of enhanced pain states.
    Authors: Soderquist RG, Milligan ED, Harrison JA, Chavez RA, Johnson KW, Watkins LR, Mahoney MJ
    J Biomed Mater Res A, 2010;93(3):1169-79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells.
    Authors: Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A
    Blood, 2010;115(9):1718-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC.
    Authors: Jindra C
    Eur J Immunol, 2010;40(2):321-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells.
    Authors: Ghirelli C, Zollinger R, Soumelis V
    Blood, 2010;115(24):5037-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
    Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
    Immunol. Lett., 2010;127(2):150-6.
  47. Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells.
    Authors: Hirosako S, Goto E, Fujii K, Tsumori K, Hirata N, Tsumura S, Kamohara H, Kohrogi H
    Clin. Exp. Immunol., 2009;155(2):266-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. IL-17 induces monocyte migration in rheumatoid arthritis.
    Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
    J. Immunol., 2009;182(6):3884-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Human cytomegalovirus suppresses type I interferon secretion by plasmacytoid dendritic cells through its interleukin 10 homolog.
    Authors: Chang WL, Barry PA, Szubin R, Wang D, Baumgarth N
    Virology, 2009;390(2):330-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA.
    Authors: Kinoshita H, Takai T, Le TA, Kamijo S, Wang XL, Ushio H, Hara M, Kawasaki J, Vu AT, Ogawa T, Gunawan H, Ikeda S, Okumura K, Ogawa H
    J. Allergy Clin. Immunol., 2009;123(1):179-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. IL-10 suppresses calcium-mediated costimulation of receptor activator NF-kappa B signaling during human osteoclast differentiation by inhibiting TREM-2 expression.
    Authors: Park-Min KH, Ji JD, Antoniv T, Reid AC, Silver RB, Humphrey MB, Nakamura M, Ivashkiv LB
    J. Immunol., 2009;183(4):2444-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008;38(4):1012-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells.
    Authors: van der Vliet HJ, Wang R, Yue SC, Koon HB, Balk SP, Exley MA
    J. Immunol., 2008;180(11):7287-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.
    Authors: Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P
    J. Immunol., 2008;181(4):2772-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  55. Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps.
    Authors: Kato A, Peters A, Suh L, Carter R, Harris KE, Chandra R, Conley D, Grammer LC, Kern R, Schleimer RP
    J. Allergy Clin. Immunol., 2008;121(6):1385-92, 1392.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  56. Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
    Authors: Rojahn A, Brusletto B, Ovstebo R, Haug KB, Kierulf P, Brandtzaeg P
    Crit. Care Med., 2008;36(9):2583-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  57. Evidence for HIV-associated B cell exhaustion in a dysfunctional memory B cell compartment in HIV-infected viremic individuals.
    Authors: Moir S, Ho J, Malaspina A, Wang W, DiPoto AC, O'Shea MA, Roby G, Kottilil S, Arthos J, Proschan MA, Chun TW, Fauci AS
    J. Exp. Med., 2008;205(8):1797-805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  58. Crucial role of the protein C pathway in governing microvascular inflammation in inflammatory bowel disease.
    Authors: Scaldaferri F, Sans M, Vetrano S, Graziani C, De Cristofaro R, Gerlitz B, Repici A, Arena V, Malesci A, Panes J, Grinnell BW, Danese S
    J. Clin. Invest., 2007;117(7):1951-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. IL-10 inhibits endothelium-dependent T cell costimulation by up-regulation of ILT3/4 in human vascular endothelial cells.
    Authors: Gleissner CA, Zastrow A, Klingenberg R, Kluger MS, Konstandin M, Celik S, Haemmerling S, Shankar V, Giese T, Katus HA, Dengler TJ
    Eur. J. Immunol., 2007;37(1):177-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  61. IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
    Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
    J. Immunol., 2007;178(5):2872-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  62. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  63. Aberrant regulation of synovial T cell activation by soluble costimulatory molecules in rheumatoid arthritis.
    Authors: Wan B, Nie H, Liu A, Feng G, He D, Xu R, Zhang Q, Dong C, Zhang JZ
    J. Immunol., 2006;177(12):8844-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  64. Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism.
    Authors: Venet F, Pachot A, Debard AL, Bohe J, Bienvenu J, Lepape A, Powell WS, Monneret G
    J. Immunol., 2006;177(9):6540-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. Selective regulation of IL-10 signaling and function by zymosan.
    Authors: Du Z, Kelly E, Mecklenbrauker I, Agle L, Herrero C, Paik P, Ivashkiv LB
    J. Immunol., 2006;176(8):4785-92.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  66. Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
    Authors: Pound JD, Batth BK, Johannessen I, Wood K
    J. Immunol., 2005;174(5):3015-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  67. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  68. Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13.
    Authors: Fujitsu Y, Fukuda K, Kimura K, Seki K, Kumagai N, Nishida T
    Invest. Ophthalmol. Vis. Sci., 2005;46(3):797-802.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  69. Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells.
    Authors: Hsieh FH, Sharma P, Gibbons A, Goggans T, Erzurum SC, Haque SJ
    Proc. Natl. Acad. Sci. U.S.A., 2005;102(40):14380-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  70. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  71. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA Standard
  72. A soluble form of the triggering receptor expressed on myeloid cells-1 modulates the inflammatory response in murine sepsis.
    Authors: Gibot S, Kolopp-Sarda MN, Bollaert PE, Lozniewski A, Mory F, Levy B, Faure GC
    J. Exp. Med., 2004;200(11):1419-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  73. A soluble form of the triggering receptor expressed on myeloid cells-1 modulates the inflammatory response in murine sepsis.
    Authors: Gibot S, Kolopp-Sarda MN, Bollaert PE, Lozniewski A, Mory F, Levy B, Faure GC
    J. Exp. Med., 2004;200(11):1419-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  74. IFN-alpha priming results in a gain of proinflammatory function by IL-10: implications for systemic lupus erythematosus pathogenesis.
    Authors: Sharif MN, Tassiulas I, Hu Y, Mecklenbrauker I, Tarakhovsky A, Ivashkiv LB
    J. Immunol., 2004;172(10):6476-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  75. BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone.
    Authors: Moreaux J, Legouffe E, Jourdan E, Quittet P, Reme T, Lugagne C, Moine P, Rossi JF, Klein B, Tarte K
    Blood, 2004;103(8):3148-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  76. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
    Authors: Chang WL, Baumgarth N, Yu D, Barry PA
    J. Virol., 2004;78(16):8720-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  77. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004;25(10):1652-9.
    Species: N/A
    Sample Types: N/A
    Applications: Standard
  78. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  79. Serum inactivation contributes to the failure of stromal-derived factor-1 to block HIV-I infection in vivo.
    Authors: Villalba S, Salvucci O, Aoki Y, De La Luz Sierra M, Gupta G, Davis D, Wyvill K, Little R, Yarchoan R, Tosato G
    J. Leukoc. Biol., 2003;74(5):880-8.
    Species: N/A
    Sample Types: N/A
    Applications: Ctrl
  80. Novel variants of the IL-10 receptor 1 affect inhibition of monocyte TNF-alpha production.
    Authors: Gasche C, Grundtner P, Zwirn P, Reinisch W, Shaw SH, Zdanov A, Sarma U, Williams LM, Foxwell BM, Gangl A
    J. Immunol., 2003;170(11):5578-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  81. Stimulation of T cell autoreactivity by anomalous expression of NKG2D and its MIC ligands in rheumatoid arthritis.
    Authors: Groh V, Bruhl A, El-Gabalawy H, Nelson JL, Spies T
    Proc. Natl. Acad. Sci. U.S.A., 2003;100(16):9452-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  82. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
    Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2002;282(5):L1049-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  83. Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
    Authors: Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A
    J. Immunol., 2002;169(10):5660-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  84. The reduction of in vitro radiation-induced Fas-related apoptosis in CD34+ progenitor cells by SCF, FLT-3 ligand, TPO, and IL-3 in combination resulted in CD34+ cell proliferation and differentiation.
    Authors: Drouet M, 49153, Mathieu J, Grenier N, Multon E, Sotto JJ, Herodin F
    Circulating NOD1 Activators and Hematopoietic NOD1 Contribute to Metabolic Inflammation and Insulin Resistance, 1999;17(5):273-85.
    Species: Primate - Baboon
    Sample Types: Whole Cells
    Applications: Bioassay
  85. Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF.
    Authors: Fadok, V A, Bratton, D L, Konowal, A, Freed, P W, Westcott, J Y, Henson, P M
    J Clin Invest, 1998;101(4):890-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  86. Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells.
    Authors: Du M, Guo P, Piao H, Wang S, Sun C, Jin L, Tao Y, Li Y, Zhang D, Zhu R, Fu Q, Li D
    J Immunol, 0;192(4):1502-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human IL-10 Protein
By Anonymous on 10/08/2018
Application: In vitro bioactivity in cell culture

Recombinant Human IL-10 Protein
By Anonymous on 09/20/2018
Application: Stem/Immune cell maintenance or differentiation

I used recombinant human IL-10 protein to polarize mouse macrophage to anti-inflammatory phenotype. Gene expression studies confirmed the significant increase in M2 genes and a decrease in the expression of M1 genes.


Recombinant Human IL-10 Protein
By Anonymous on 04/02/2018
Application: In vivo study

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By Anonymous on 05/16/2017
Application: Cell proliferation

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By Anonymous on 01/21/2016
Application: In vitro bioactivity in cell culture