Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF

Catalog # Availability Size / Price Qty
10426-AV-050
Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein Binding Activity.
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Recombinant Cynomolgus Integrin alpha V beta 3 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Recombinant Human Vitronectin (Catalog # 2308-VN) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Integrin alpha V beta 3 (Catalog # 10426-AV) binds with an ED50 of 0.06-0.72 μg/mL.

Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Integrin alpha V beta 3 protein
Cynomolgus Monkey Integrin alpha V
(Phe31-Val992)
Accession # XP_005573729.1
His-Pro2x GGGSGGGS-Acidic Tail 6-His tag
Cynomolgus Monkey Integrin beta 3
(Gly27-Asp718)
Accession # XP_005584667.1
His-Pro2x GGGSGGGS-Basic tailHA tag
N-terminusC-terminus
N-terminal Sequence
Analysis
Phe31 (Integrin alpha V) & Gly27 (Integrin beta 3)
Predicted Molecular Mass
115 kDa (Integrin alpha V) & 86 kDa (Integrin beta 3)
SDS-PAGE
105-160 kDa, under reducing conditions

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10426-AV

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10426-AV

Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Data Images

Binding Activity Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein Binding Activity. View Larger

When Recombinant Human Vitronectin (Catalog # 2308-VN) is immobilized at 2 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey Integrin aV beta 3 (Catalog # 10426-AV) binds with an ED50 of 0.06-0.72 µg/mL.

SDS-PAGE Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein SDS-PAGE. View Larger

2 μg/lane of Recombinant Cynomolgus Monkey Integrin alpha V beta 3 Protein (Catalog # 10426-AV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-160 kDa.

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Background: Integrin alpha V beta 3

Integrin alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1­3). The non­covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2­4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N­terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta­propeller structure (4). The alpha V subunit contributes a "thigh and a calf" region, while the beta 3 subunit contains a PSI domain and four cysteine­rich I­EGF folds. The alpha V subunit domains termed thigh, calf­1 and calf­2 generate a "knee" region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by "inside out" signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Within the extracellular domain (ECD), cynomolgus alpha V shares 99% amino acid (aa) sequence identity with the 962 aa human alpha V, while cynomolgus beta 3 ECD shares almost 100% aa sequence identity with the 692 aa human beta 3 ECD. Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone, as well as promotes their apoptosis (8, 9). M­CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Cell entry of several viruses is mediated by alpha V beta 3 (4, 10). alpha V beta 3 is involved in several other signaling pathways by direct interaction with receptor tyrosine kinases and ligands. For example, it cooperates with endothelial cell VEGF R2 in angiogenesis, and with IGF­1 to promote cancer cell proliferation and invasiveness (11, 12).

References
  1. Hynes, R. O. (2002) Cell 110:673.
  2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
  3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
  4. Xiong, J. et al. (2001) Science 294:339.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:69.
  8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
  9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
  11. Somanath, P.R. et al. (2009) Angiogenesis 12:177.
  12. Saegusa, J. et al. (2009) J. Biol. Chem. 284:24106.
Entrez Gene IDs
3685 (Human)
Alternate Names
antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V beta 3; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; Vitronectin receptor subunit alpha; VNRADKFZp686A08142

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