Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10226-LR-050
Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF Binding Activity
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Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Cynomolgus Monkey LAIR1 Fc Chimera binds with an ED50 of 1‑6 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey LAIR1 protein
Gln22-His163
Accession #
N-terminal Sequence
Analysis
Gln22, deduced from deblocked sequence at Glu23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
42 kDa
SDS-PAGE
58-64 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10226-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Bovine Collagen I is coated at 10 μg/mL, 100 μL/well, Recombinant Cynomolgus LAIR1 Fc Chimera (Catalog # 10226-LR) binds with an ED50 of 1‑6 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus LAIR1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-64 kDa and 110-130 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). Cynomolgus LAIR-1 cDNA encodes a 296 amino acid (aa) polypeptide precursor. Based on the similarity with human LAIR1, it is likely to be a type I transmembrane (TM) protein containing a 21 aa signal sequence, a 144 aa extracellular domain (ECD), a 21 aa TM domain and a 110 aa cytoplasmic domain. Within ECD cynomolgus LAIR-1 shares 82% aa identity with human LAIR-1. LAIR-1 ECD includes one C2-type Ig-like domain. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). LAIR1 shows high-affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6).

References
  1. Meyaard, L.(2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1997) Immunity 7:283.
  3. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535.
  4. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828.
  5. Verbrugge, A. et al. (2003) Int. Immunol. 15:1349.
  6. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.
Long Name
Leukocyte-associated Ig-like Receptor 1
Entrez Gene IDs
3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)
Alternate Names
CD305 antigen; CD305; CD305leukocyte-associated Ig-like receptor 1; HLAIR1; LAIR1; LAIR-1; leukocyte-associated immunoglobulin-like receptor 1

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