Recombinant Cynomolgus LAIR1 Fc Chimera Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Bovine Collagen I is coated at 10 μg/mL, 100 μL/well, Recombinant Cynomolgus LAIR1 Fc Chimera (Catalog # 10226-LR) binds with an ED50 of 1‑6 ng/mL.
2 μg/lane of Recombinant Cynomolgus LAIR1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-64 kDa and 110-130 kDa, respectively.
Leukocyte-associated Ig-like receptor-1 (LAIR1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). Cynomolgus LAIR-1 cDNA encodes a 296 amino acid (aa) polypeptide precursor. Based on the similarity with human LAIR1, it is likely to be a type I transmembrane (TM) protein containing a 21 aa signal sequence, a 144 aa extracellular domain (ECD), a 21 aa TM domain and a 110 aa cytoplasmic domain. Within ECD cynomolgus LAIR-1 shares 82% aa identity with human LAIR-1. LAIR-1 ECD includes one C2-type Ig-like domain. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and down-regulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). LAIR1 shows high-affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6).
- Meyaard, L.(2003) J. Biol. Regul. Homeost. Agents 17:330.
- Meyaard, L. et al. (1997) Immunity 7:283.
- Lebbink, R.J. et al. (2004) J. Immunol. 172:5535.
- Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828.
- Verbrugge, A. et al. (2003) Int. Immunol. 15:1349.
- Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.
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