Recombinant Cynomolgus Monkey B7-H3 Protein, CF

Catalog # Availability Size / Price Qty
9426-B3-100
Recombinant Cynomolgus Monkey B7-H3 Protein, CF Bioactivity
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Recombinant Cynomolgus Monkey B7-H3 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey B7-H3 protein
Gly27 & Leu29-Thr461, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gly27 & Leu29
Predicted Molecular Mass
48 kDa (Gly27) & 47 kDa (Leu29)
SDS-PAGE
65 - 80 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9426-B3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity View Larger

Recombinant Cynomolgus Monkey B7‑H3 inhibits IFN-gamma secretion by human T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-10 μg/mL.

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Background: B7-H3

Cynomolgus Monkey B7 homolog 3 (B7-H3), also known as CD276 antigen, is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses (1-3). Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C-like domains with a short cytoplasmic tail. Among the family members there is about
20‑40% amino acid (aa) sequence identity. Cynomolgus monkey B7-H3 shares approximately 97% and 88% aa sequence identity with human and mouse B7-H3 respectively. B7-H3 mRNA is found in various normal tissues and in several tumor cell lines, but is not detectable in peripheral blood mononuclear cells (PBMCs). Inflammatory cytokines, such as IFN gamma, and a combination of phorbol myristate acetate (PMA) and ionomycin induce expression of B7-H3 protein on dendritic cells (DCs) and monocytes (4). The receptor(s) for B7-H3 remains unknown. Both murine and human B7-H3 fusion proteins fail to bind to resting T cells, but can recognize activated T cells which stimulated with PHA or ConA, indicating that B7-H3 receptors are induced upon T cell activation (5). B7-H3 was reported to act as a
co-stimulatory regulator to enhance the proliferation of both CD4+ and CD8+ T cells, the induction of cytotoxic T cells, and IFN-gamma production in the presence of TCR signaling (1). B7‑H3 was also reported to play an inhibitory role on T-cell activation. The inhibition may govern through nuclear factor of activated T cells (NFAT),
NF‑ kappa B, and AP-1 factors, three major signaling pathways through which TCR regulates gene transcription, which suggesting that B7‑H3 might have more than one receptor on T cells (6). B7-H3 protein expresses on a wide variety of cancers, including stomach, lung, prostate, kidney, ovary, pancreas, liver, bladder and breast. The role of B7-H3 in anti-tumor immunity is controversial, because B7-H3 has conflicting costimulatory and coinhibitory functions (7).

References
  1. Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
  2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J.Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Kyung H. Yi and Lieping Chen. (2009) Immunol Rev. 229:145.
  5. Sun M, et al. (2002) J Immunol. 168:6294.
  6. Hofmeyer KA, et al. (2008) Proc Natl Acad Sci USA. 105:10277.
  7. Ni L and Dong C. (2017) Immunol Rev. 276:52.
Long Name
B7 Homolog 3
Entrez Gene IDs
80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names
B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule

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