Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10197-CD-050
Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein, CF Binding Activity
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Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey CD160 Fc Chimera that produces 50% of the optimal binding response 1.5-9 ng/mL
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD160 protein
Cynomolgus Monkey CD160
(Ile27-Ser159)
Accession # EHH50231
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ile27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41 kDa
SDS-PAGE
40-60 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10197-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL, Recombinant Cynomolgus Monkey CD160Fc Chimera (Catalog # 10197-CD) binds with an ED50 of 1.5-9 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey CD160 Fc Chimera (Catalog # 10197-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-60 kDa and 80-120 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD160

CD160, also known as Natural killer cell receptor BY55, is a GPI-anchored member of the Ig superfamily that is expressed on both cytolytic lymphocytes and some unstimulated CD4+ T cells (1-4). In human, it is expressed principally on non-myeloid hematopoietic cells (1, 5-7). It is synthesized as a preproprotein with 181 amino acids including a 24 amino acid (aa) signal sequence, a 135 aa CD160 chain that contain one 96 aa V‑type Ig‑like domain, and a 22 aa propeptide that is cleaved to generate a GPI‑linkage at Ser159. The GPI‑linked CD160 is known to be cleaved by phospholipases and generate an 80 kDa (presumably trimeric) band in SDS‑PAGE (1, 8).  Mature cynomolgus CD160 shares 91% aa sequence identity with human CD160. CD160 is known to bind to HLA-G1, HLA-C, and HVEM (6, 9, 10). Upon engagement, it is reported to associate with CD2 in cis under certain conditions (11, 12). The effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to HLA-G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on CD56DIM NK cells, CD160 signaling in response to HLA-C binding promotes IFN-gamma, TNF-alpha, and IL-6 secretion (10). When up-regulated on CD4+ T cells following activation, CD160 engagement by HVEM (expressed by APC) serves to block a simultaneous LIGHT stimulation of HVEM that promotes receptor expression and cytokine release (1, 2, 7, 13).

References
  1. Cai, G. & G.J. Freeman (2009) Immunol. Rev. 229:244.
  2. del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
  3. Maiza, H. et al. (1993) J. Exp. Med. 178:1121.
  4. Anumanthan, A. et al. (1998) J. Immunol. 161:2780.
  5. Abecassis, S. et al. (2007) J. Invest. Dermatol. 127:1161.
  6. Fons, P. et al. (2006) Blood 108:2608.
  7. Kaye, J. et al. (2008) Nat. Immunol. 9:122.
  8. Giustiniani, J. et al. (2007) J. Immunol. 178:1293.
  9. Giustinani, J. et al. (2009) J. Immunol. 182:63.
  10. Barakonyi, A. et al. (2004) J. Immunol. 173:5349.
  11. Nikolova, M. et al. (2002) Int. Immunol. 14:445.
  12. Rabot, M. et al. (2006) Transpl. Immunol. 17:36.
  13. Cai, G. et al. (2008) Nat. Immunol. 9:176.
Entrez Gene IDs
11126 (Human); 54215 (Mouse); 502585 (Rat); 102117412 (Cynomolgus Monkey)
Alternate Names
BY55; BY55FLJ46513; CD160 antigenimmunoglobulin superfamily member; CD160 molecule; CD160; Natural killer cell receptor BY55; NK1; NK28

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