Recombinant Cynomolgus Monkey CD160 Fc Chimera Protein, CF Summary
|Cynomolgus Monkey CD160 |
Accession # EHH50231
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 μg/mL, Recombinant Cynomolgus Monkey CD160Fc Chimera (Catalog # 10197-CD) binds with an ED50 of 1.5-9 ng/mL.
2 μg/lane of Recombinant Cynomolgus Monkey CD160 Fc Chimera (Catalog # 10197-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-60 kDa and 80-120 kDa, respectively.
CD160, also known as Natural killer cell receptor BY55, is a GPI-anchored member of the Ig superfamily that is expressed on both cytolytic lymphocytes and some unstimulated CD4+ T cells (1-4). In human, it is expressed principally on non-myeloid hematopoietic cells (1, 5-7). It is synthesized as a preproprotein with 181 amino acids including a 24 amino acid (aa) signal sequence, a 135 aa CD160 chain that contain one 96 aa V‑type Ig‑like domain, and a 22 aa propeptide that is cleaved to generate a GPI‑linkage at Ser159. The GPI‑linked CD160 is known to be cleaved by phospholipases and generate an 80 kDa (presumably trimeric) band in SDS‑PAGE (1, 8). Mature cynomolgus CD160 shares 91% aa sequence identity with human CD160. CD160 is known to bind to HLA-G1, HLA-C, and HVEM (6, 9, 10). Upon engagement, it is reported to associate with CD2 in cis under certain conditions (11, 12). The effects of CD160 ligation appear to be context dependent. When expressed on endothelial cells, CD160 binding to HLA-G1 initiates apoptosis, and thus impacts angiogenesis (6). When expressed on CD56DIM NK cells, CD160 signaling in response to HLA-C binding promotes IFN-gamma, TNF-alpha, and IL-6 secretion (10). When up-regulated on CD4+ T cells following activation, CD160 engagement by HVEM (expressed by APC) serves to block a simultaneous LIGHT stimulation of HVEM that promotes receptor expression and cytokine release (1, 2, 7, 13).
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