Recombinant Cynomolgus Monkey CD58/LFA-3 Fc Protein, CF

R&D Systems | Catalog # 11432-CD

Fc Chimera, Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems CHO-derived Recombinant Cynomolgus Monkey CD58/LFA-3 Fc Protein (11432-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

XP_0055542263.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD58/LFA-3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD58/LFA-3 protein
Cynomolgus Monkey CD58
(Ile29-Arg215)
Accession # XP_005542263.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile29

Predicted Molecular Mass

48 kDa

SDS-PAGE

70-78 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Cynomolgus Monkey CD58/LFA‑3 Fc Chimera (Catalog # 11432-CD) binds to recombinant Human CD2 Fc Chimera Protein (Catalog # 1856-CD) with a ED50 of 0.150-2.00 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey CD58/LFA-3 Fc Protein, CF

Recombinant Cynomolgus Monkey CD58/LFA‑3 Fc Chimera Protein SEC-MALS.

Recombinant Cynomolgus Monkey CD58/LFA‑3 Fc Chimera Protein (Catalog # 11432-CD) has a molecular weight (MW) of 121.4 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Cynomolgus Monkey CD58/LFA‑3 Fc Chimera Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Cynomolgus Monkey CD58/LFA-3 Fc Chimera Protein (Catalog # 11432-CD) binds to recombinant Human CD2 Fc Chimera Protein (1856-CD) with a ED50 of 0.150‑2.00 μg/mL.

Recombinant Cynomolgus Monkey CD58/LFA‑3 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey CD58/LFA‑3 Fc Chimera Protein (Catalog # 11432-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-78 kDa and 140-160 kDa, respectively.

Formulation, Preparation, and Storage

11432-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD58/LFA-3

T cells require a signal induced by the engagement of the T cell receptor and a "co-stimulatory" signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3),  belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2-4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58 and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4). Blockade of CD58-CD2 interaction on NK cells resulted in diminished production of IFN-gamma and TNF-alpha in response to Human cytomegalovirus (HCMV)-infected cells (7). Expression of CD58 was significantly increased in anisomycin-treated hepatocellular carcinoma (HCC), and may be critical player in NK-mediated immunotherapeutic effects (8).

References

  1. Davis, S.J. and van der Merwe P.A. (1996) Immunol. Today 17:177.
  2. Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893.
  3. Bolhuis, R.L. et al. (1986) J. Immunol. 136:3939.
  4. Crawford, K. et al. (2003) Blood 102:1745.
  5. Kanner, S.B. et al. (1992) J. Immunol. 148:2023.
  6. Bullens, D.M. et al. (2001) International Immunol. 13:181.
  7. Rölle A. et al. (2016) Eur J Immunol. 46:2420
  8. Kim M. et al. (2018) Sci Rep. 8:10668.
 

Alternate Names

CD58, LFA-3, LFA3

Entrez Gene IDs

965 (Human); 102123839 (Cynomolgus Monkey); 710732 (Rhesus Macaque)

Gene Symbol

CD58

UniProt

XP_0055542263.1

Additional CD58/LFA-3 Products

Product Documents for Recombinant Cynomolgus Monkey CD58/LFA-3 Fc Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus Monkey CD58/LFA-3 Fc Protein, CF

For research use only

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