Recombinant Human CD2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1856-CD-050
Recombinant Human CD2 Fc Chimera Protein, CF SDS-PAGE
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Recombinant Human CD2 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD58 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human CD2 Fc Chimera binds with an ED50 of 1-5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human CD2 protein
Human CD2
(Lys25-Asp209)
Accession # P06729
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Lys25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48 kDa
SDS-PAGE
59-65 kDa, reducing conditions

Product Datasheets

1856-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE Recombinant Human CD2 Fc Chimera Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human CD2 Fc chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 59-65 kDa and 120-130 kDa, respectively.

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Background: CD2

T cell surface antigen CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T cells, NK cells, B cells and some antigen presenting cells. CD2 functions as an activator of T lymphocytes and thymocytes (1). Mature human CD2 consists of a 185 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 116 aa cytoplasmic tail. The extracellular domain is composed of two immunoglobulin-superfamily domains with highly-charged binding regions (2). Within the ECD, human CD2 shares 47% and 48% aa sequence identity with mouse and rat CD2, respectively. It interacts with both CD58 and CD59 directly to activate T cells and their adhesion pathways (3). The conformational flexibility of the CD2 molecule affects function through the conformational status of the adhesive ligands (4). Together with PSTPIP1, CD2 works to regulate T cell activation (5). These two proteins colocalize with a second CD2-binding protein to signal immunological synapse formation in T cells (6). CD2 is an adhesion molecule present on the cell surface of T cells, natural killer (NK) cells, and B cells; and its interaction with CD58 on antigen-presenting cells plays an important role in their immune reaction (7). CD2-CD58 interactions play a critical role in the anti-tumor immune response, and restoration of this signaling is an important strategy for anti-tumor therapy (8). Furthermore, CD2-CD58 interactions are pivotal for the activation and function of adaptive natural killer cells in human cytomegalovirus infection (9).

References
  1. Sayre, P. et al. (1987). Proc. Natl. Acad. Sci. 84:2941.
  2. Bodian, DL. et al. (1994) Structure. 2:755.
  3. Hahn, W. C. et al. (1992). Science. 256:1805.
  4. Yang, J. J. et al. (2001). Curr. Prot. Pept. Sci. 2:1.
  5. Yang, H. and Reinherz, EL. (2006). J. Immunol. 176:5898.
  6. Badour, K. et al. (2003). Immunity. 18:141.
  7. Kingma, D.W. et al. (2002) Cytometry 50:243.
  8. Nishikori, M. et al. (2016) J Clin Cell Immunol 7:406.
  9. Rolle, A. et al. (2016) Eur. J. Immunol. 46:2420.
Entrez Gene IDs
914 (Human); 12481 (Mouse)
Alternate Names
CD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5

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