Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera Protein, CF

Datasheet / CoA / SDS
Product Details
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey CTLA‑4 Fc Chimera is coated at 0.5 µg/mL, 100 μL/well, Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) binds with an ED50 of 5-30 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Cynomolgus Monkey CTLA-4
    Accession # XP_005574071
    IEGRMD Human IgG1
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    40 kDa
    46-57 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Image
When Recombinant Cynomolgus Monkey CTLA‑4 FcChimera (Catalog # 9336-CT) is coated at 0.5 µg/mL,100 μL/well, Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) binds with anED50 of 5-30 ng/mL.
Background: CTLA-4
CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Cyno CTLA-4 has a 98% homology to human CTLA-4. Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41-43 kDa (2). Within the ECD, human CTLA-4 shares 98%, 68%, 71% and 83-86% aa sequence identity with cynomolgus, mouse, rat and porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3-5). In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1-2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11-13).
  • References:
    1. Harper, K. et al. (1991) J. Immunol. 147:1037.
    2. Teft, W.A. et al. (2006) Annu. Rev. Immunol. 24:65.
    3. Magistrelli, G. et al. (1999) Eur. J. Immunol. 29:3596.
    4. Tector, M. et al. (2009) BMC Immunol. 10:51.
    5. Oaks, M.K. and K.M. Hallett (2000) J. Immunol. 164:5015.
    6. Vijayakrishnan, L. et al. (2004) Immunity 20:563.
    7. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
    8. Jansson, A. et al. (2005) J. Immunol 175:1575.
    9. Darlington, P.J. et al. (2005) J. Immunol. 175:996.
    10. Platt, A.M. et al. (2010) J. Immunol. 185:1558.
    11. Wing, K. et al. (2008) Science. 322: 271.
    12. Friedline, R.H. et al. (2009) J. Exp. Med. 206:421.
    13. Jain, N. et al. (2010) Proc. Natl. Acad. Sci. USA. 107:1524.
  • Long Name:
    Cytotoxic T-lymphocyte-associated Molecule 4
  • Entrez Gene IDs:
    1493 (Human); 12477 (Mouse); 403696 (Canine); 102115124 (Cynomolgus Monkey)
  • Alternate Names:
    CD; CD152 antigen; CD152; CD152IDDM12; CD28; celiac disease 3; CELIAC3; CTLA4; CTLA-4; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte-associated serine esterase-4; GRD4; GSE; ICOS; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
Related Research Areas


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Have you used Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order


Customer Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

R&D Systems Guarantee