Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF

R&D Systems | Catalog # 10366-ER

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein (10366-ER)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

XP_005549616.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all EGFR Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey EGFR protein
Cynomolgus Monkey EGFR
(Leu25-Ser645)
Accession # XP_005549616.1
 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

95 kDa

SDS-PAGE

110-130 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey EGFR Fc Chimera (Catalog # 10366-ER) is immobilized at 2 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human EGF that produces 50% of the optimal binding response is found to be approximately 4-20 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein Binding Activity

Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein Binding Activity

When Recombinant Cynomolgus Monkey EGFR Fc Chimera (Catalog # 10366-ER) is immobilized at 2 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human EGF that produces 50% of the optimal binding response is found to be approximately 4-20 ng/mL.
Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein SDS-PAGE

Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey EGFR Fc Chimera (Catalog # 10366-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa and 220-260 kDa, respectively.

Formulation, Preparation, and Storage

10366-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: EGFR

Epidermal growth factor receptor (EGFR) is a member of a subfamily of receptor tyrosine kinases comprised of four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD) containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3, 4). The mature ECD of cynomolgus EGFR shares 99% and 90% amino acid sequence identity with human and mouse EGFR, respectively. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (5‑7). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (5-7). EGFR is overexpressed in a wide variety of tumors and is the target of several anti-cancer drugs (8).

References

  1. Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
  2. Shilo, B.Z. (2005) Development 132:4017.
  3. Guillaudeau, A. et al. (2012) PLoS One. 7:1.
  4. Reiter J.L. et al. (2001) Genomics 71:1.
  5. Freed, D.M. et al. (2017) Cell. 171:683.
  6. Burgess, A.W. et al. (2003) Mol. Cell 12:541.
  7. Faria, J.A. et al. (2016) BBRC. 478:39.
  8. Lee, C.K. et al. (2017) J. Thoracic Oncology. 12:403.

Long Name

Epidermal Growth Factor Receptor

Alternate Names

EGF R, ErbB, ErbB1, HER-1

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Gene Symbol

EGFR

UniProt

XP_005549616.1

Additional EGFR Products

Product Documents for Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF

For research use only

Citations for Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF

Customer Reviews for Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Cynomolgus Monkey EGFR Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes