Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF

Name: Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF
Brand: R&D Systems
SKU: 10215-CL
Price: 579 USD
Availability: InStock

R&D Systems | Catalog # 10215-CL

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Citations (1)

Product Documents

GS Linker, Defined heterodimer stoichiometry.

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems HEK293-derived Recombinant Cynomolgus Monkey IL-12 (linked heterodimer) (10215-CL)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

NP_001274204 (IL-12p40) & XP_005546300 (IL-12p35)

Structure / Form

Disulfide-linked heterodimer

Applications

Bioactivity

View all IL-12 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey IL-12 protein

Cynomolgus Monkey IL-12p40 (Ile23-Ser328) Accession # NP_001274204	GGGSGGGSGGGS	Cynomolgus Monkey IL-12p35 (Arg57-Ser253)Accession # XP_005546300
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile23

Predicted Molecular Mass

58 kDa

SDS-PAGE

65-78 kDa, under reducing conditions

Activity

Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells.
The ED₅₀ for this effect is 0.15-0.9 ng/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey IL-12 (linked heterodimer), CF

Recombinant Cynomolgus Monkey IL-12 (linked heterodimer) Bioactivity

Recombinant Cynomolgus Monkey IL-12 (Catalog # 10215-CL) enhances IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 0.15-0.9 ng/mL.

Recombinant Cynomolgus Monkey IL-12 (linked heterodimer) SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey IL-12 (10215-CL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-78 kDa.

Formulation, Preparation, and Storage

10215-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-12

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit (1, 2). The cynomolgus monkey p35 subunit shares 93.4% sequence identity with the human homolog, and the p40 subunit shares 96.4% identity with its human homolog. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation (2, 3). Current evidence indicates that IL-12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent (1-3).