Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF

Catalog # Availability Size / Price Qty
2523-BP-050
Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF Bioactivity
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Recombinant Cynomolgus Monkey IL-18 BPa His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.015-0.075 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-18 BPa protein
Thr28-Arg207, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr28
Structure / Form
Monomer
Predicted Molecular Mass
20 kDa
SDS-PAGE
54-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2523-BP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Cynomolgus Monkey IL‑18 BPa inhibits Recombinant Human IL-18-induced IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.015-0.075 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey IL‑18 BPa was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 54-60 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18 BPa

Cynomolgus Interleukin 18 binding protein (IL-18 BP) is a 20 kDa secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity (1). IL-18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. Human IL-18BP encodes for at least four isoforms by alternative splicing. The IL-18 BP isoform a and c each contains one immunoglobulin (Ig)-like C2-type domain while isoform b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins (2). Cynomolgus IL-18BPa shares 83% sequence identity with human and 62% with mouse IL-18BPa, respectively. Several poxviruses also encode proteins with sequence similarity to IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP (3).

References
  1. Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
  2. Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
  3. Calderara, S. et al. (2001) Virology 279:22.
Long Name
Interleukin 18 Binding Protein Isoform a
Entrez Gene IDs
10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)
Alternate Names
IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa

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