Recombinant Cynomolgus Monkey TIM-4 Fc Chimera Protein, CF Summary
|Cynomolgus Monkey TIM-4
Accession # XP_005558436
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Cynomolgus Monkey TIM-4 Fc Chimera (Catalog # 9384-TM) inhibits anti-CD3-induced proliferation of human T cells. The ED50 for this effect is 0.2-1.2 μg/mL.
TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is an approximately 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1-3). Sequence alignment with the human TIM-4 shows that mature cynomolgus monkey TIM-4 has a predicted 280 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 43 aa cytoplasmic tail. Within the ECD, cynomolgus monkey TIM-4 shares 87 % and 48 % aa sequence identity with human and mouse TIM-4, respectively. Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1or TIM-3. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (2, 3). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (3, 4). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (3, 5). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (3).
- Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
- Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494.
- Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
- McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
- Khademi, M. et al. (2004) J. Immunol. 172:7169.
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