Recombinant Cynomolgus Monkey TIM-4 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.2-1.2 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived
    Cynomolgus Monkey TIM-4
    (Glu25-Gln305)
    Accession # XP_005558436
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Glu25
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    57 kDa
  • SDS-PAGE
    95-112 kDa, reducing conditions
9384-TM
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
RecombinantCynomolgus Monkey TIM-4 Fc Chimera(Catalog # 9384-TM) inhibits anti-CD3-induced proliferation of human Tcells. The ED50 for this effect is
0.2-1.2 μg/mL.
Background: TIM-4
TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is an approximately 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1-3). Sequence alignment with the human TIM-4 shows that mature cynomolgus monkey TIM-4 has a predicted 280 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 43 aa cytoplasmic tail. Within the ECD, cynomolgus monkey TIM-4 shares 87 % and 48 % aa sequence identity with human and mouse TIM-4, respectively. Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1or TIM-3. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (2, 3). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (3, 4). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (3, 5). The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (3).
  • References:
    1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
    2. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
    3. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
    4. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
    5. Khademi, M. et al. (2004) J. Immunol. 172:7169.
  • Long Name:
    T Cell Immunoglobulin Mucin-4
  • Entrez Gene IDs:
    91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)
  • Alternate Names:
    FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4
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