Recombinant Cynomolgus Monkey uPAR Fc Chimera Protein, CF Summary
|Cynomolgus Monkey UPAR|
Accession # Q9GK78.1
|IEGRMD||Human IgG1 Fc|
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 1.00 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Cynomolgus Monkey UPAR Fc Chimera (Catalog # 10972-UK) is immobilized at 1.00 μg/mL (100 µL/well), Recombinant Human u-Plasminogen Activator/Urokinase (1310-SE) binds with an ED50 of 15.0-90.0 ng/mL.
2 μg/lane of Recombinant Cynomolgus Monkey uPAR Fc Chimera (Catalog # 10972-UK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-85 kDa and 150-170 kDa, respectively.
Urokinase plasminogen activator receptor (uPAR), also known as CD87, is a cysteine-rich cell surface glycoprotein belonging to the Ly6/uPAR (LU) superfamily and is involved in the activation of plasminogen to plasmin (1). Mature uPAR consists of an extracellular domain (ECD) with 3 LU domains (D1, D2, and D3) similar to Ly‑6 antigens and snake venom alpha -neurotoxins and a C-terminal glycosyl-phosphatidylinositol (GPI) anchor linked to the last residue of the third LU domain (1-3). The ECD of mature cynomolgus uPAR shares 95% amino acid sequence identity with human uPAR. Posttranslational modification generates several soluble uPAR fragments including uPAR, uPAR DIIDIII, and uPAR DI in human (1-3). The urokinase-type Plasminogen Activator (uPA) is one of two activators that converts the extracellular zymogen plasminogen to plasmin. The binding of uPAR with uPA initiates a proteolytic cascade resulting in the degradation of extracellular matrix components and stimulates tissue remodeling (4). The uPAR/uPA interaction also initiates signal transduction responses resulting in activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis (4). uPAR can interact with integrins to suppress normal integrin adhesive function and promote adhesion to vitronectin through a high affinity vitronectin binding site (5, 6). uPAR expression is limited in normal human tissue but is highly expressed by diverse cancer cells and by non-malignant cells that infiltrate cancers and uPAR expression is associated with poor prognosis and increased risk of metastasis (7). uPAR is considered as a biomarker in many inflammatory diseases including cancer, cardiovascular diseases, chronic kidney diseases and diabetes (6).
- Blasi, F. and Carmeliet, P. (2002) Nat Rev Mol. Cell Biol 3:932.
- Leth, J.M. and Ploug, M. (2021) Front Cell Dev. Biol. 9:732015.
- Behrendt, N. et al. (1996) J Biol. Chem. 271:22885.
- Mahmood, N. et al. (2018) Front. Oncol. 8:24.
- Smith, H. et al. (2010) Nat Rev Mol Cell Biol 11:23.
- Desmedt, S. et al. (2017) Crit. Rev. Clin. Lab. Sci. 54:117.
- Mazar, A.P. (2008) Clin. Cancer Res. 14:5649.
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