Recombinant Cynomolgus PD-1 Fc Chimera Protein, CF

R&D Systems | Catalog # 8578-PD

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Cynomolgus PD-1 Fc Chimera Protein (8578-PD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

NP_001271065

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey PD-1 protein
Cynomolgus Monkey PD-1
(Leu25-Gln167)
Accession # NP_001271065		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu25

Predicted Molecular Mass

43 kDa (monomer)

SDS-PAGE

57-68 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human PD-L2/B7-DC Fc Chimera (Catalog # 1224-PL) is coated at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey PD-1 Fc Chimera that produces 50% optimal binding response is 0.03-0.15 μg/mL.

Reviewed Applications

Read 3 reviews rated 5 using 8578-PD in the following applications:

Formulation, Preparation, and Storage

8578-PD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature Cynomolgus monkey PD-1 consists of a 148 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The Cynomolgus monkey PD-1 ECD shares 95% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 and PD-L2 (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while
PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13).

References

  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  5. Watanabe, N et al. (2003) Nat. Immunol. 4:670.
  6. Zhang, X. et al. (2004) Immunity 20:337.
  7. Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483.
  8. Nishimura, H et al. (1996) Int. Immunol. 8:773.
  9. Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677.
  10. Butte, M.J. et al. (2007) Immunity 27:111.
  11. Okazaki, T. et al. (2013) Nat. Immunol. 14:1212.
  12. Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293.
  13. Nogrady, B. (2014) Nature 513:S10.

Long Name

Programmed Death-1

Alternate Names

CD279, PD1, PDCD1, SLEB2

Entrez Gene IDs

5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)

Gene Symbol

PDCD1

UniProt

NP_001271065

Additional PD-1 Products

Product Documents for Recombinant Cynomolgus PD-1 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus PD-1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Cynomolgus PD-1 Fc Chimera Protein, CF

Customer Reviews for Recombinant Cynomolgus PD-1 Fc Chimera Protein, CF (3)

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3 Customer Ratings
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  • Recombinant Cynomolgus PD-1 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Cell Adhesion
    Verified Customer | Posted 10/24/2018
  • Recombinant Cynomolgus PD-1 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Media additive for protein or antibody production
    Verified Customer | Posted 10/12/2018
  • Recombinant Cynomolgus PD-1 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 07/11/2018

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