Recombinant Cynomolgus/Rhesus EDAR Fc Chimera Protein, CF Summary
|Cynomolgus Monkey/Rhesus Macaque EDAR|
Accession # XP_005575284.1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque EDAR Fc Chimera Protein (Catalog # 10968-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-65 kDa and 110-130 kDa, respectively.
EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region; the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Based on its high homology with human EDAR, cynomologus EDAR is predicted to be a 488 amino acid (aa) protein with a 26 aa signal, a 163 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The cynomolgus and human EDAR homologs share 99% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2, which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.
- Headon, D.J. and P.A. Overbeek (1999) Nat.Genet. 22:370.
- Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
- Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
- Schneider, P. et al. (2001) J. Biol. Chem. 276:18819.
- Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
- Yan, M. et al. (2000) Science 290:523.
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