Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His Protein, CF

Name: Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His Protein, CF
Brand: R&D Systems
SKU: 10451-EP
Price: 487 USD
Availability: InStock

R&D Systems | Catalog # 10451-EP

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His Protein (10451-EP)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

NP_001035118.1

Applications

Bioactivity

View all EpCAM/TROP1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived EpCAM/TROP1 protein
Gln24-Lys265, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln24; inferred from deblocking reaction revealing Lys25

Predicted Molecular Mass

28 kDa

SDS-PAGE

30-43 kDa, under reducing conditions.

Activity

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.
The ED₅₀ for this effect is 0.3-1.5 μg/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His Protein, CF

Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His-tag Protein Bioactivity.

Immobilized Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His Protein (Catalog # 10451-EP) enhances L Cells mouse fibroblast adhesion in the presence of Human Fibronectin. The ED₅₀ for this effect is 0.3-1.5 μg/mL.

Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus/Rhesus EpCAM/TROP1 His-tag Protein (Catalog # 10451-EP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-43 kDa.

Formulation, Preparation, and Storage

10451-EP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein. Based on its similarity with human EpCAM, cynomolgus/rhesus EpCAM is predicted to consist of a 242 amino acid (aa) extracellular domain with two epidermal growth factor like (EGF like) repeats within the cysteine rich N terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Cynomolgus and human EpCAM share 93% aa sequence identity (1). During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is expressed on basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia but not on normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). It is additionally expressed on undifferentiated embryonic stem cells, thymocytes, and dendritic cells (3-5). It is up-regulated on actively proliferating epithelial tissues, during adult liver regeneration, and on many epithelial cell-derived carcinomas (2, 6). EpCAM functions as a homophilic cell adhesion molecule (7). It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1 (8-11) that can interfere with cell adhesion (12, 13). Proteolytic cleavage of EpCAM releases multiple fragments from the ECD as well as a cytoplasmic fragment that can regulate gene transcription (14-16).