Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein, CF Summary
|Cynomolgus Monkey BTLA|
Accession # XP_005548224
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 µg/mL, Recombinant Cynomolgus Monkey BTLA FcChimera binds with an ED50 of 25-125 ng/mL.
2 μg/lane of Recombinant Cynomolgus Monkey BTLA was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 48-58 kDa and 100-115 kDa, respectively.
B- and T-lymphocyte attenuator (BTLA; CD272) is a 50-57 kDa type I transmembrane glycoprotein in the CD28 family of T cell co-stimulatory molecules (1-3). Mature cynomolgus BTLA contains a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane sequence, and a 111 aa cytoplasmic domain. In humans, the cytoplasmic tail transmits inhibitory signaling via two ITIM motifs and three Tyr phosphorylation sites (4, 5). The ECD of cynomolgus BTLA shares 88% aa identity with that of human BTLA. A splice variant lacking the transmembrane domain has been reported in humans (6). Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type, and BTLA does not form homodimers (7). BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands (8). BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells (9). Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature naïve cells (9). BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR cross-linking (10). Under the control of ROR gamma t and IL-7, BTLA regulates the homeostasis and inflammatory responses of gamma δT cells (11). The binding of BTLA and HVEM does not preclude the concurrent binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha (12).
- Murphy, K.M. et al. (2006) Nat. Rev. Immunol. 6:671.
- Croft, M. (2005) Trends. Immunol. 26:292.
- Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
- Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
- Chemnitz, J.M. et al. (2006) J. Immunol. 176:6603.
- Han, P. et al. (2004) J. Immunol. 172:5931
- Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
- Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
- Hurchla, M.A. et al. (2005) J. Immunol. 174:3377.
- Krieg, C. et al. (2007) Nat. Immunol. 8:162.
- Bekiaris, V. et al. (2013) Immunity 39:1082.
- Cai G and Freeman GJ, (2009) Immunol Rev. 229:244
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein, CF and earn rewards!
Have you used Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image