Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein, CF

R&D Systems | Catalog # 10030-BT

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein (10030-BT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

XP_005548224

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived BTLA protein
Cynomolgus Monkey BTLA
(Met1-Leu150)
Accession # XP_005548224		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys31

Predicted Molecular Mass

40.3 kDa

SDS-PAGE

48-58 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 µg/mL, Recombinant Cynomolgus Monkey BTLA Fc Chimera binds with an ED50 of 25-125 ng/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein, CF

Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein Binding Activity

Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein Binding Activity

When Recombinant Mouse HVEM/TNFRSF14 Fc Chimera (Catalog # 2516-HV) is immobilized at 0.5 µg/mL, Recombinant Cynomolgus Monkey BTLA Fc Chimera binds with an ED50 of 25-125 ng/mL.
Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein SDS-PAGE

Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus Monkey BTLA was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-58 kDa and 100-115 kDa, respectively.

Formulation, Preparation, and Storage

10030-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTLA

B- and T-lymphocyte attenuator (BTLA; CD272) is a 50-57 kDa type I transmembrane glycoprotein in the CD28 family of T cell co-stimulatory molecules (1-3). Mature cynomolgus BTLA contains a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane sequence, and a 111 aa cytoplasmic domain. In humans, the cytoplasmic tail transmits inhibitory signaling via two ITIM motifs and three Tyr phosphorylation sites (4, 5). The ECD of cynomolgus BTLA shares 88% aa identity with that of human BTLA. A splice variant lacking the transmembrane domain has been reported in humans (6). Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type, and BTLA does not form homodimers (7). BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands (8). BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells (9). Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature naïve cells (9). BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR cross-linking (10). Under the control of ROR gamma t and IL-7, BTLA regulates the homeostasis and inflammatory responses of gamma delta T cells (11). The binding of BTLA and HVEM does not preclude the concurrent binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha (12).

References

  1. Murphy, K.M. et al. (2006) Nat. Rev. Immunol. 6:671.
  2. Croft, M. (2005) Trends. Immunol. 26:292.
  3. Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
  4. Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
  5. Chemnitz, J.M. et al. (2006) J. Immunol. 176:6603.
  6. Han, P. et al. (2004) J. Immunol. 172:5931
  7. Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
  8. Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
  9. Hurchla, M.A. et al. (2005) J. Immunol. 174:3377.
  10. Krieg, C. et al. (2007) Nat. Immunol. 8:162.
  11. Bekiaris, V. et al. (2013) Immunity 39:1082.
  12. Cai G and Freeman GJ, (2009) Immunol Rev. 229:244

Long Name

B- And T-Lymphocyte Attenuator

Alternate Names

CD272

Entrez Gene IDs

151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)

Gene Symbol

BTLA

UniProt

XP_005548224

Additional BTLA Products

Product Documents for Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein, CF

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Product Specific Notices for Recombinant Cynomolgus/Rhesus Macaque BTLA Fc Protein, CF

For research use only

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