Recombinant Cynomolgus TIM-1/KIM-1/HAVCR Fc Chimera, CF

Catalog # Availability Size / Price Qty
9676-TM-100
Recombinant Cynomolgus TIM-1/KIM-1/HAVCR Fc Chimera, CF Bioactivity
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Recombinant Cynomolgus TIM-1/KIM-1/HAVCR Fc Chimera, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.4-2.4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey TIM-1/KIM-1/HAVCR protein
Cynomolgus Monkey TIM-1/KIM-1/HAVCR
(Val18-Thr253)
Accession # BAJ61042
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val18, Asp20, & Val22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52 kDa
SDS-PAGE
91-107 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9676-TM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Cynomolgus Monkey TIM‑1/KIM‑1/HAVCR Fc Chimera (Catalog # 9676-TM) inhibits anti-CD3-induced proliferation of PHA-activated human T cells. The ED50for this effect is 0.4-2.4 μg/mL.

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Background: TIM-1/KIM-1/HAVCR

T cell immunoglobulin and mucin domain 1 (TIM-1), also known as KIM-1 and HAVcr1, is a member of the TIM family which is involved in the regulation of innate and adaptive immune responses (1). TIM-1 is a type I transmembrane protein that contains an N-terminal immunoglobulin-like domain, a mucin domain with O- and N-linked carbohydrates, a transmembrane segment, and a cytoplasmic signaling domain (2). Multiple TIM-1 variants can be produced due to polymorphisms or alternative splicing resulting in deletions in the mucin domain. TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule (3). TIM-1 is expressed on splenic B cells, IL-10+ regulatory B cells, CD4+ T cells, mast cells, invariant NKT (iNKT) cells, dendritic cells, kidney epithelium and a broad range of mucosal epithelium (1, 4-6). It is up-regulated on activated Th2 cells, after dendritic cell maturation, and on kidney tubular epithelial cells after injury (7-10). Within the extracellular domain, Cynomolgus monkey TIM-1 shares 69% and 44% amino acid sequence identity with human and mouse TIM-1, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (2, 11). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (12). TIM-1 ligation induces T cell proliferation and promotes cytokine production (12).

References
  1. Du, P. et al. (2016) J. Immunol. Res. 2016:8605134.
  2. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  3. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  4. Ding, Q. et al. (2011) J. Clin. Invest. 121:3645.
  5. Ma, J. et al. (2011) Biochem. Biophys. Res. Commun. 406:223.
  6. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
  7. Kuehn, E.W. et al. (2002) Am. J. Physiol. Renal Physiol. 283:F1326.
  8. Umetsu, S.E. et al. (2005) Nat. Immunol. 6:447.
  9. Xiao, S. et al. (2011) Eur. J. Immunol. 41:1539.
  10. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
  11. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  12. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

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