Recombinant Feline CD8 alpha Protein, CF

Name: Recombinant Feline CD8 alpha Protein, CF
Brand: R&D Systems
SKU: 9649-CD
Price: 724 USD
Availability: InStock

R&D Systems | Catalog # 9649-CD

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

R&D Systems NS0-derived Recombinant Feline CD8 alpha Protein (9649-CD)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P41688

View all CD8 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived feline CD8 protein
Ala22-Tyr188, with a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

19 kDa

SDS-PAGE

28-31 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9649-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD8

CD8 is a dimeric complex made up of two Ig superfamily members (1). The CD8 alpha chain is a type I transmembrane glycoprotein that can dimerize with either itself forming CD8 alpha alpha, or with a 30-35 kDa CD8 beta chain, forming CD8 alpha beta (2). CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR co-receptor, while CD8 alpha alpha promotes T cell survival and differentiation (1, 3). The mature feline CD alpha consists of a 167 amino acid (aa) extracellular domain (ECD), and runs at ~30 kDa on reduced SDS-PAGE. Within the ECD, feline CD8 alpha shares 58%, 47%, 51%, and 69% amino acid sequence identity with human, mouse, porcine, and canine CD8 alpha, respectively.