Recombinant Human 4-1BB/TNFRSF9 His-tag Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human 4-1BB/TNFRSF9/CD137 is immobilized at 50 ng/mL, 100 μL/well, the concentration of Recombinant Human 4‑1BB Ligand/TNFSF9 (Catalog # 2295-4L) that produces 50% of the optimal binding response is approximately 2.5-12.5 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Leu24-His183, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu24
  • Predicted Molecular Mass
    18 kDa
  • SDS-PAGE
    25-38 kDa, reducing conditions
9220-4B
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human
4-1BB/TNFRSF9(Catalog # 9220-4B) is coated at 50 ng/mL, Recombinant Human4-1BB Ligand/TNFSF9 Recombinant Human 4‑1BB Ligand/
TNFSF9 (Catalog # 2295-4L) binds with a ED50 of
2.5-15 ng/mL.
Background: 4-1BB/TNFRSF9/CD137
4-1BB, also known as CD137 and TNFRSF9, is an approximately 30 kDa transmembrane glycoprotein in the TNF receptor superfamily. 4-1BB functions in the development and activation of multiple immune cells (1). Mature human 4-1BB consists of a 163 amino acid (aa) extracellular domain (ECD) with four TNFR cysteine-rich repeats, a 27 aa transmembrane segment, and a 42 aa cytoplasmic domain (2, 3). Within the ECD, human 4-1BB shares 60% aa sequence identity with mouse and rat 4-1BB. 4-1BB is expressed as a disulfide-linked homodimer on various populations of activated T cell including CD4+, CD8+, memory CD8+, NKT, and regulatory T cells (4-7) as well as on myeloid and mast cell progenitors, dendritic cells, mast cells, and bacterially infected osteoblasts (8-11). It binds with high affinity to the transmembrane 4-1BB Ligand/TNFSF9 which is expressed on antigen presenting cells and myeloid progenitor cells (3, 8). This interaction costimulates the proliferation, activation, and/or survival of the 4-1BB expressing cell (3-7). It can also enhance the activation-induced cell death of repetitively stimulated T cells (3). Mice lacking 4-1BB show augmented T cell activation, perhaps due to its absence on regulatory T cells (12). 4-1BB can associate with OX40 on activated T cells, forming a complex that responds to either ligand and inhibits Treg and CD8+ T cell proliferation (13). Reverse signaling through 4-1BB Ligand inhibits the development of dendritic cells, B cells, and osteoclasts (8, 11) but supports mature dendritic cell survival and costimulates the proliferation and activation of mast cells (9, 10).
4-1BB activation enhances CD8+ T cell and NK cell mediated anti-tumor immunity (14). It also contributes to the development of inflammation in high fat diet-induced metabolic syndrome (15). Soluble forms of 4-1BB and 4-1BB Ligand circulate at elevated levels in the serum of rheumatoid arthritis and hematologic cancer patients, respectively (16, 17).
  • References:
    1. Wang, C. et al. (2009) Immunol. Rev. 229:192.
    2. Schwarz, H. et al. (1993) Gene 134:295.
    3. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
    4. Wen, T. et al. (2002) J. Immunol. 168:4897.
    5. Pulle, G. et al. (2006) J. Immunol. 176:2739.
    6. Zheng, G. et al. (2004) J. Immunol. 173:2428.
    7. Kim, D. et al. (2008) J. Immunol. 180:2062.
    8. Lee, S. et al. (2008) Nat. Immunol. 9:917.
    9. Choi, B.K. et al. (2009) J. Immunol. 182:4107.
    10. Nishimoto, H. et al. (2005) Blood 106:4241.
    11. Saito, K. et al. (2004) J. Biol. Chem. 279:13555.
    12. Lee, S. et al. (2005) J. Immunol. 174:6803.
    13. Ma, B.Y. et al. (2005) Blood 106:2002.
    14. Choi, B.K. et al. (2010) J. Immunol. 185:1404.
    15. Kim, C. et al. (2011) Diabetes 60:3159.
    16. Michel, J. et al. (1998) Eur. J. Immunol. 28:290.
    17. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
  • Entrez Gene IDs:
    3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)
  • Alternate Names:
    4-1BB ligand receptor; 41BB; 4-1BB; 4-1BB/TNFRSF9/CD137; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9
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