Protein C (PROC) is a vitamin K-dependent serine protease synthesized in liver as a single-chain precursor (1). The N-terminus consists of a signal peptide (aa 1-32) and a propeptide (aa 33-42). The mature chain (aa 43-461) is converted to two disulfide-linked chains (light: aa 43-199 and heavy: 200‑461) and both forms are inactive. The light chain consists of Gla (gamma-carboxy-glutamate) domain and two EGF-like domains. The heavy chain consists of an activation peptide (aa 200‑211) and serine protease domain (aa 212-450). Present in plasma at 3 to 5 mg/L, PROC plays a key role in anticoagulation. Physiologically, the inactive forms of PROC are converted to the active form by thrombin, which releases the activation peptide. The active PROC cleaves factors VIIIa and Va to inactivate them. This anticoagulation activity can be enhanced by a presence of a cofactor such as Protein S. In hereditary thrombophilia, PROC deficiency is caused by a genetic mutation which affects PROC activity. A severe recessive form may result in massive thrombosis fatal to patient.
Recombinant Human Active Coagulation Factor XIV/ProteinC, CF
R&D Systems | Catalog # 4998-SE
Key Product Details
- R&D Systems CHO-derived Recombinant Human Active Coagulation Factor XIV/ProteinC (4998-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
Ala43-Pro461, with a C-terminal 10-His tag
The isolated protein was activated by thrombin/thrombomodulin complex and further purified.
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC (Catalog # ES011).
The specific activity is >275 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
4998-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Coagulation Factor XIV/Protein C
References
- Shen, L. and Dahlbäck, B. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1673.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional Coagulation Factor XIV/Protein C Products
Product Documents for Recombinant Human Active Coagulation Factor XIV/ProteinC, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Active Coagulation Factor XIV/ProteinC, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Human Active Coagulation Factor XIV/ProteinC, CF (4998-SE):
- Assay Buffer: 50 mM Tris, 0.15 M NaCl, 10 mM CaCl2, 0.01% (w/v) Brij 35, pH 8.5
- Recombinant Human Active Coagulation Factor XIV/Protein C (rhPROC) (Catalog # 4998-SE)
- Substrate: Boc-Val-Pro-Arg-AMC (Catalog # ES011)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhPROC to 2 ng/µL in Assay Buffer.
- Dilute Substrate to 1 mM in Assay Buffer.
- Load into plate 50 µL of 2 ng/µL rhPROC, and start the reaction by adding 50 µL of 1 mM Substrate. As a Substrate Blank combine 50 µL of 1 mM Substrate and 50 µL of Assay Buffer.
- Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-Amino, 4-Methyl Coumarin (AMC) (Sigma, Catalog # A-9891).
Per Well:
- rhPROC: 0.1 µg
- Substrate: 500 μM
Associated Pathways
