Recombinant Human AgRP Protein Summary
Ala21-Thr132 & Leu47-Thr132, both with a C-terminal 6-His tag
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.|
|Reconstitution||Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuropeptide that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) on MC-3 and MC-4 receptors (1-4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature human AgRP is a 112 amino acid (aa) peptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Human AgRP shares 82% and 72% aa sequence identity with mouse and rat AgRP, respectively. It shares 32% aa sequence identity with Agouti. As in the case of Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is up-regulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones in a mechanism that does not involve melanocortin receptors (14).
- Martin, N.M. et al. (2006) Peptides 27:333.
- Fan, W. et al. (1997) Nature 385:165.
- Ollmann, M.M. et al. (1997) Science 278:135.
- Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
- Shutter, J.R. et al. (1997) Genes Develop. 11:593.
- Kiefer, L.L. et al. (1998) Biochemistry 37:991.
- Jackson, P.J. et al. (2002) Biochemistry 41:7565.
- Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
- Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
- Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
- Small, C.K. et al. (2001) Diabetes 50:248.
- Vink, T. et al. (2001) Mol. Psychiatry 6:325.
- Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
- Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265-266:108.
Citations for Recombinant Human AgRP Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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MC4R-dependent suppression of appetite by bone-derived lipocalin 2
Authors: I Mosialou, S Shikhel, JM Liu, A Maurizi, N Luo, Z He, Y Huang, H Zong, RA Friedman, J Barasch, P Lanzano, L Deng, RL Leibel, M Rubin, T Nicholas, W Chung, LM Zeltser, KW Williams, JE Pessin, S Kousteni
Sample Types: Whole Cells
Effects of estradiol on cerebrospinal fluid levels of agouti-related protein in ovariectomized rhesus monkeys.
Authors: Xiao E, Kim AJ, Dutia R
Sample Types: N/A
Applications: HPLC Standard
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