Recombinant Human Amyloid Precursor Protein Fc Chimera, CF

Isoform APP695
Catalog # Availability Size / Price Qty
9937-AP-100
Recombinant Human Amyloid Precursor Protein Fc Chimera, CF Binding Activity
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Recombinant Human Amyloid Precursor Protein Fc Chimera, CF Summary

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Contactin-3 (Catalog # 9800-CN) is coated at 2 µg/mL, 100 μL/well, Recombinant Human APP695 Fc Chimera binds with an ED50 of 7-42 ng/mL
Source
Mouse myeloma cell line, NS0-derived human APP695 protein
Human Amyloid-beta A4 (Isoform APP695)
(Leu18-Lys612)
Accession # P05067-4
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Leu18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
94 kDa
SDS-PAGE
111-133 kDa, reducing conditions

Product Datasheets

9937-AP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Human Amyloid Precursor Protein Fc Chimera, CF Binding Activity View Larger

When Recombinant Mouse Contactin-3 (Catalog # 9800-CN) iscoated onto a microplate at 2 µg/mL, Recombinant Human APP695 Fc Chimera binds with an ED50 of 7-42 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: APP695

Amyloid precursor protein (APP) is a type I membrane protein with several isoforms due to alternative splicing. Of the three major splice isoforms of APP (APP695, APP751, and APP770) APP695 is the predominant neuronal form from which Amyloid beta peptide  and transcriptionally-active cleaved intracellular domain of APP (AICD) are preferentially generated by selective processing through the amyloidogenic pathway (1). Human APP695 consists of a 17 amino acid (aa) signal sequence, a 607 aa extracellular domain (ECD), a 24 aa transmembrane domain, and a 47 aa cytoplasmic domain. Within the ECD, human APP695 shares 97% aa sequence identity with mouse and rat APP695. Amyloid beta is a major molecule implicated in pathogenesis of Alzheimer's disease (AD) and related dementias (2). AICD regulates expression by direct promoter binding of multiple genes, including APP itself, the beta-secretase, BACE-1 and the Amyloid beta-degrading enzyme, Neprilysin (3, 1). As such, APP695 plays an important role in brain development, learning and memory, synaptic plasticity, and neurodegeneration including AD (4).

References
  1. Nalivaeva, N.N. and Turner A. J. (2013) FEBS Lett. 587:2046.
  2. Haass, C. (2004) EMBO J. 23:483.
  3. Passer, B. et al. (2000) J. Alzheimers Dis. 2:289.
  4. Benilova, I. et al. (2012) Nat. Neurosci. 15:349.
Long Name
Amyloid Precursor Protein 695
Entrez Gene IDs
351 (Human); 11820 (Mouse)
Alternate Names
APP695

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