Recombinant Human Amyloid Precursor Protein Fc Chimera, CF

R&D Systems | Catalog # 9937-AP

Isoform APP695
R&D Systems
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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Amyloid Precursor Protein Fc Chimera (9937-AP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P05067-4

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all APP695 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human APP695 protein
Human Amyloid-beta A4 (Isoform APP695)
(Leu18-Lys612)
Accession # P05067-4		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu18

Predicted Molecular Mass

94 kDa

SDS-PAGE

111-133 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Contactin-3 (Catalog # 9800-CN) is coated at 2 µg/mL, 100 μL/well, Recombinant Human APP695 Fc Chimera binds with an ED50 of 7-42 ng/mL

Scientific Data Images for Recombinant Human Amyloid Precursor Protein Fc Chimera, CF

Recombinant Human Amyloid Precursor Protein Fc Chimera Binding Activity

Recombinant Human Amyloid Precursor Protein Fc Chimera Binding Activity

When Recombinant Mouse Contactin-3 (Catalog # 9800-CN) is coated onto a microplate at 2 µg/mL, Recombinant Human APP695 Fc Chimera binds with an ED50 of 7-42 ng/mL.

Formulation, Preparation, and Storage

9937-AP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: APP695

Amyloid precursor protein (APP) is a type I membrane protein with several isoforms due to alternative splicing. Of the three major splice isoforms of APP (APP695, APP751, and APP770) APP695 is the predominant neuronal form from which Amyloid beta peptide  and transcriptionally-active cleaved intracellular domain of APP (AICD) are preferentially generated by selective processing through the amyloidogenic pathway (1). Human APP695 consists of a 17 amino acid (aa) signal sequence, a 607 aa extracellular domain (ECD), a 24 aa transmembrane domain, and a 47 aa cytoplasmic domain. Within the ECD, human APP695 shares 97% aa sequence identity with mouse and rat APP695. Amyloid beta is a major molecule implicated in pathogenesis of Alzheimer's disease (AD) and related dementias (2). AICD regulates expression by direct promoter binding of multiple genes, including APP itself, the beta-secretase, BACE-1 and the Amyloid beta-degrading enzyme, Neprilysin (3, 1). As such, APP695 plays an important role in brain development, learning and memory, synaptic plasticity, and neurodegeneration including AD (4).

References

  1. Nalivaeva, N.N. and Turner A. J. (2013) FEBS Lett. 587:2046.
  2. Haass, C. (2004) EMBO J. 23:483.
  3. Passer, B. et al. (2000) J. Alzheimers Dis. 2:289.
  4. Benilova, I. et al. (2012) Nat. Neurosci. 15:349.

Long Name

Amyloid Precursor Protein 695

Entrez Gene IDs

351 (Human); 11820 (Mouse)

Gene Symbol

APP

UniProt

P05067-4

Additional APP695 Products

Product Documents for Recombinant Human Amyloid Precursor Protein Fc Chimera, CF

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Product Specific Notices for Recombinant Human Amyloid Precursor Protein Fc Chimera, CF

For research use only

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