Recombinant Human Angiopoietin-like 1 Flag-tag Protein, CF Summary
Gly24-Asp491, with a C-terminal Flag-tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS with Trehalose.|
|Reconstitution||Reconstitute at 250 μg/mL in water.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human Integrin a1 beta 1 (Catalog # 7064-AB) is used at 1 µg/mL, Recombinant Human Angiopoietin-like Protein 1/ANGPTL1 Flag-tag (Catalog # 10013-AN) binds with an ED50 of 8-80 ng/mL.
2 μg/lane of Recombinant Human Angiopoietin‑like Protein 1/ANGPTL1 Flag-tag (Catalog# 10013-AN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a predominant band at 60 kDa and 180 kDa, respectively.
Background: Angiopoietin-like Protein 1/ANGPTL1
Angiopoietin like Protein 1 (ANGPTL1), also known as Angioarrestin, ARP1 and ANG3, is a secreted glycoprotein that contains a coiled coil domain at the N-terminus and a fibrinogen like domain at the C-terminus (1). ANGPTL1 is one of the seven members of the Angiopoietin-like family proteins. Similar to angiopoietins, ANGPTL1 secretes as a disulfide linked homo-oligomer (2-4). Mature human ANGPTL1 shares 94% sequence identity with its mouse homolog. ANGPTL1 is highly expressed in adrenal gland, placenta, thyroid gland, heart, skeletal muscle and small intestine, and weakly expressed in testis, ovary, colon, pancreas, kidney and stomach (1). Expression of ANGPTL1 is down-regulated in various cancers (4-6). ANGPTL1 has anti-angiogenic functions, inhibiting endothelial cell proliferation, migration, tube formation, and adhesion (4, 5). ANGPTL1 is a tumor suppressor. Over-expression of ANGPTL1 inhibits tumor growth (4, 5), migration, and metastasis (6, 7). Upon binding to integrin alpha 1/ beta 1, the FAK/Src-JAK-STAT3 signaling pathway is inhibited in hepatocellular carcinoma, promoting apoptosis and suppressing cancer invasion and angiogenesis (6). Binding to integrin alpha 1/ beta 1 also leads to the inhibition of FAK/ERK/SP1 signaling pathway to induce the expression of micro RNA 630, which suppresses the zinc finger protein SLUG to inhibit lung cancer cell mobility (7). ANGPT-L1 has been shown to directly interact with and antagonize MET receptor activity in hepatocellular carcinoma (8).
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- Dhanabal, M. et al. (2002) Cancer Res 62:3834.
- Smagur, A. et al. (2005) Acta Biochim Pol. 52:875.
- Yan, Q. et al. (2017) Cancer Res. 77:5831.
- Kuo, T-C. et al. (2013) J Clin Invest 123:1082.
- Chen H-A, et al. (2016) Hematology 64(5):1637.
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