Recombinant Human Angiopoietin-like 1 Flag-tag Protein, CF

Catalog # Availability Size / Price Qty
10013-AN-050
Recombinant Human Angiopoietin-like 1 Flag-tag Protein, CF Binding Activity
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Recombinant Human Angiopoietin-like 1 Flag-tag Protein, CF Summary

Purity
>75%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Integrin alpha 1 beta 1 Recombinant Human Integrin  alpha 1 beta 1 (Catalog # 7064-AB) is used at 1 μg/mL, Recombinant Human Angiopoietin‑like Protein 1/ANGPTL1 binds with an ED50 of 8-80 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human Angiopoietin-like Protein 1/ANGPTL1 protein
Gly24-Asp491, with a C-terminal Flag-tag
Accession #
N-terminal Sequence
Analysis
Arg22
Structure / Form
Disulfide-linked oligomer
Predicted Molecular Mass
56 kDa
SDS-PAGE
Predominant band at 60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10013-AN

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human Integrin  alpha 1 beta 1 (Catalog # 7064-AB) is used at 1 μg/mL, Recombinant Human Angiopoietin‑like Protein 1/ANGPTL1 Flag-tag (Catalog # 10013-AN) binds with an ED50 of 8‑80 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Angiopoietin‑like Protein 1/ANGPTL1 Flag-tag (Catalog# 10013-AN) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing a predominant band at 60 kDa and 180 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 1/ANGPTL1

Angiopoietin like Protein 1 (ANGPTL1), also known as Angioarrestin, ARP1 and ANG3, is a secreted glycoprotein that contains a coiled coil domain at the N-terminus and a fibrinogen like domain at the C-terminus (1). ANGPTL1 is one of the seven members of the Angiopoietin-like family proteins. Similar to angiopoietins, ANGPTL1 secretes as a disulfide linked homo-oligomer (2-4). Mature human ANGPTL1 shares 94% sequence identity with its mouse homolog. ANGPTL1 is highly expressed in adrenal gland, placenta, thyroid gland, heart, skeletal muscle and small intestine, and weakly expressed in testis, ovary, colon, pancreas, kidney and stomach (1). Expression of ANGPTL1 is down-regulated in various cancers (4-6). ANGPTL1 has anti-angiogenic functions, inhibiting endothelial cell proliferation, migration, tube formation, and adhesion (4, 5). ANGPTL1 is a tumor suppressor. Over-expression of ANGPTL1 inhibits tumor growth (4, 5), migration, and metastasis (6, 7). Upon binding to integrin alpha 1/ beta 1, the FAK/Src-JAK-STAT3 signaling pathway is inhibited in hepatocellular carcinoma, promoting apoptosis and suppressing cancer invasion and angiogenesis (6). Binding to integrin alpha 1/ beta 1 also leads to the inhibition of FAK/ERK/SP1 signaling pathway to induce the expression of micro RNA 630, which suppresses the zinc finger protein SLUG to inhibit lung cancer cell mobility (7). ANGPT-L1 has been shown to directly interact with and antagonize MET receptor activity in hepatocellular carcinoma (8).

References
  1. Kim, I. et al. (1999), FEBS Lett 443:353
  2. Kim, K-T. et al. (2005) J. Biol. Chem. 280:20126.
  3. Procopio, W. N., et al. (1999) J. Biol. Chem. 274:30196
  4. Dhanabal, M. et al. (2002) Cancer Res 62:3834.
  5. Smagur, A. et al. (2005) Acta Biochim Pol. 52:875.
  6. Yan, Q. et al. (2017) Cancer Res. 77:5831.
  7. Kuo, T-C. et al. (2013) J Clin Invest 123:1082.
  8. Chen H-A, et al. (2016) Hematology 64(5):1637.
Entrez Gene IDs
9068 (Human); 72713 (Mouse); 679942 (Rat)
Alternate Names
ANG-3; ANG3angioarrestin; Angiopoietin like Protein 1; angiopoietin Y1; angiopoietin-3; angiopoietin-like 1; Angiopoietin-like Protein 1; angiopoietin-related protein 1; ANGPT3; ANGPTL1; AngY; AngY1; ARP1; ARP1angiopoietin 3; dJ595C2.2 (angiopoietin Y1); dJ595C2.2; KIAA0351; UNQ162

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