Recombinant Human Axl His-tag Protein, CF

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Recombinant Human Axl His-tag Protein Binding Activity
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Recombinant Human Axl His-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human Axl-His-tag (Catalog # 10448-AL) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human GAS6 (Catalog # 885-GSB) binds with an ED50 of 0.1-0.5 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human Axl protein
Glu33-Trp450, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Glu 33
Predicted Molecular Mass
46 kDa
74-82 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human Axl His-tag Protein Binding Activity View Larger

When Recombinant Human Axl His-tag (Catalog # 10448-AL) is immobilized at 0.25 µg/mL (100 µL/well), it binds to Recombinant Human Gas6 (Catalog # 885-GSB) with an ED50 of 0.1-0.5 ng/mL.

SDS-PAGE Recombinant Human Axl His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human Axl His-tag Protein (Catalog# 10448-AL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-82 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Axl

Axl, also known as Ufo and Ark, is a widely expressed 140 kDa glycoprotein in the TAM receptor tyrosine kinase family. TAM family receptors (Dtk/Tyro3, Axl, and Mer) are involved in regulation of the inflammatory response, cell survival and migration, and tumorigenesis (1). Mature human Axl consists of a 426 aa extracellular domain (ECD) that contains two Ig-like domains and two fibronectin type III domains, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain that includes the tyrosine kinase domain (2). Within the ECD, human Axl shares approximately 82% aa sequence identity with mouse and rat Axl. A short alternately spliced form of human Axl has a 9 aa deletion in the extracellular juxtamembrane region (2). Axl binds the vitamin K-dependent protein Gas6 which triggers tyrosine autophosphorylation of the Axl cytoplasmic domain (3). Activation of Axl induces a broad range of activities including platelet aggregation and thrombus formation (4), macrophage and dendritic cell phagocytosis of apoptotic cells (5), NK cell development from hematopoietic progenitor cells (6), and in vivo angiogenesis (7). Axl is highly expressed in solid cancers and promotes in vivo tumorigenesis and tumor cell invasiveness (7, 8). It contributes to vascular remodeling and inflammatory cell infiltration in response to hypertension and restricted blood flow (9). It also functions as a cellular entry receptor for Gas6-opsonized lentiviruses (10). A 70-80 kDa soluble portion of the Axl ECD can be shed by proteolytic cleavage, and this fragment retains the ability to bind Gas6 (11, 12).

  1. Linger, R.M.A. et al. (2011) Adv. Cancer Res. 100:35.
  2. O’Bryan, J.P. et al. (1991) Mol. Cell. Biol. 11:5016.
  3. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  4. Cosemans, J.M.E.M. et al. (2010) J. Thromb. Haemost. 8:1797.
  5. Seitz, H.M. et al. (2007) J. Immunol. 178:5635.
  6. Park, I.-K. et al. (2009) Blood 113:2470.
  7. Holland, S. et al. (2005) Cancer Res. 65:9294.
  8. Rankin, E.B. et al. (2010) Cancer Res. 70:7570.
  9. Korshunov, V.A. et al. (2006) Circ. Res. 98:1446.
  10. Morizono, K. et al. (2011) Cell Host Microbe 9:286.
  11. O’Bryan, J.P. et al. (1995) J. Biol. Chem. 270:551.
  12. Ekman, C. et al. (2010) J. Thromb. Haemost. 8:838.
Long Name
Axl Receptor Tyrosine Kinase
Entrez Gene IDs
558 (Human); 26362 (Mouse); 102126361 (Cynomolgus Monkey)
Alternate Names
AI323647; ARK; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming sequence/gene; Axl; EC 2.7.10; EC; JTK11; Tyro7; tyrosine-protein kinase receptor UFO; UFO; UFOoncogene AXL


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Recombinant Human Axl His-tag Protein, CF
By Vasken Parsekhian on 12/16/2020
Application: Immunoassay Standard
Reason for Rating: Parallel with endogenous human Axl