Recombinant Human Gas6 Protein, CF

Catalog # Availability Size / Price Qty
885-GSB-050
885-GSB-500
Graph showing bioactivity of recombinant human GAS6
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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (4)

Recombinant Human Gas6 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED50 for this effect is 25-150 ng/mL
Source
Mouse myeloma cell line, NS0-derived human Gas6 protein
Ala49-Ala678, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala49
Predicted Molecular Mass
70.5 kDa
SDS-PAGE
70-85 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

885-GSB

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Citrate.
Reconstitution Reconstitute at 500 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Bioactivity of Recombinant Human Gas6 protein View Larger

Recombinant Human Gas6 885-GSB down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 25-150 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gas6

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77%-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the
EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

References
  1. Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
  2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  3. Stitt, T.N. et al. (1995) Cell 80:661.
  4. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
  5. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  6. Goruppi, S. et al. (1997) FEBS Lett. 415:59.
  7. Sather, S. et al. (2007) Blood 109:1026.
  8. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
  9. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
  10. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
  11. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
  12. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
  13. Gallicchio, M. et al. (2005) Blood 105:1970.
  14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
  15. Wu, Y. et al. (2005) J. Cell Sci. 118:539.
  16. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
Long Name
Growth-arrest-specific Protein 6
Entrez Gene IDs
2621 (Human); 14456 (Mouse)
Alternate Names
AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6

Citations for Recombinant Human Gas6 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Cabozantinib inhibits AXL- and MET-dependent cancer cell migration induced by growth-arrest-specific 6 and hepatocyte growth factor
    Authors: T Hara, A Kimura, T Miyazaki, H Tanaka, M Morimoto, K Nakai, J Soeda
    Biochem Biophys Rep, 2020;21(0):100726.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis
    Authors: S Alivernini, L MacDonald, A Elmesmari, S Finlay, B Tolusso, MR Gigante, L Petricca, C Di Mario, L Bui, S Perniola, M Attar, M Gessi, AL Fedele, S Chilaka, D Somma, SN Sansom, A Filer, C McSharry, NL Millar, K Kirschner, A Nerviani, MJ Lewis, C Pitzalis, AR Clark, G Ferracciol, I Udalova, CD Buckley, E Gremese, IB McInnes, TD Otto, M Kurowska-S
    Nat. Med., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. AXL confers cell migration and invasion by hijacking a PEAK1-regulated focal adhesion protein network
    Authors: A Abu-Thurai, MA Goyette, J Boulais, C Delliaux, C Apcher, C Schott, R Chidiac, H Bagci, MP Thibault, D Davidson, M Ferron, A Veillette, RJ Daly, AC Gingras, JP Gratton, JF Côté
    Nat Commun, 2020;11(1):3586.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Standard
  4. p5RHH nanoparticle-mediated delivery of AXL siRNA inhibits metastasis of ovarian and uterine cancer cells in mouse xenografts
    Authors: KA Mills, JM Quinn, ST Roach, M Palisoul, M Nguyen, H Noia, L Guo, J Fazal, DG Mutch, SA Wickline, H Pan, KC Fuh
    Sci Rep, 2019;9(1):4762.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. A TAZ-AXL-ABL2 Feed-Forward Signaling Axis Promotes Lung Adenocarcinoma Brain Metastasis
    Authors: JP Hoj, B Mayro, AM Pendergast
    Cell Rep, 2019;29(11):3421-3434.e8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Interaction between androgen receptor and coregulator SLIRP is regulated by Ack1 tyrosine kinase and androgen
    Authors: D De Silva, Z Zhang, Y Liu, JS Parker, C Xu, L Cai, GG Wang, HS Earp, YE Whang
    Sci Rep, 2019;9(1):18637.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET
    Authors: A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí
    Cell Mol Gastroenterol Hepatol, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. A gain-of-functional screen identifies the Hippo pathway as a central mediator of receptor tyrosine kinases during tumorigenesis
    Authors: T Azad, K Nouri, HJ Janse van, SM Maritan, L Wu, Y Hao, T Montminy, J Yu, P Khanal, LM Mulligan, X Yang
    Oncogene, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells
    Authors: DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma
    MBio, 2019;10(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Inhibition of MERTK Promotes Suppression of Tumor Growth in BRAF Mutant and BRAF Wild-Type Melanoma
    Authors: L Sinik, KA Minson, JJ Tentler, J Carrico, SM Bagby, WA Robinson, R Kami, T Burstyn-Co, SG Eckhardt, X Wang, SV Frye, HS Earp, D DeRyckere, DK Graham
    Mol. Cancer Ther., 2019;18(2):278-288.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells
    Authors: LC Cheng, YL Chen, AN Cheng, AY Lee, CY Cho, JS Huang, SE Chuang
    J. Biomed. Sci., 2018;25(1):80.
  12. Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology
    Authors: K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa
    Nat Commun, 2018;9(1):433.
    Applications: Bioassay
  13. Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
    Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
    Br. J. Cancer, 2017;117(12):1787-1797.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation
    Authors: SN Cross, JA Potter, P Aldo, JY Kwon, M Pitruzzell, M Tong, S Guller, CV Rothlin, G Mor, VM Abrahams
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  15. Inhibition of the Receptor Tyrosine Kinase AXL Restores Paclitaxel Chemosensitivity in Uterine Serous Cancer
    Authors: ML Palisoul, JM Quinn, E Schepers, IS Hagemann, L Guo, K Reger, AR Hagemann, CK McCourt, PH Thaker, MA Powell, DG Mutch, KC Fuh
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. BCL6 promotes glioma and serves as a therapeutic target
    Authors: L Xu, Y Chen, M Dutra-Clar, A Mayakonda, M Hazawa, SE Savinoff, N Doan, JW Said, WH Yong, A Watkins, H Yang, LW Ding, YY Jiang, JW Tyner, J Ching, JP Kovalik, V Madan, SL Chan, M Müschen, JJ Breunig, DC Lin, HP Koeffler
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Therapeutic activity of anti-AXL antibody against triple-negative breast cancer Patient Derived Xenografts and metastasis
    Authors: Wilhem Leconet
    Clin. Cancer Res, 2016;0(0):.
    Applications: Western Blot
  18. The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
    Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
    J. Biol. Chem., 2016;291(20):10586-601.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex
    Authors: Zhi-Min Yuan
    J Mol Cell Biol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Phosphatidylserine Sensing by TAM Receptors Regulates AKT-Dependent Chemoresistance and PD-L1 Expression.
    Authors: Kasikara C, Kumar S, Kimani S, Tsou W, Geng K, Davra V, Sriram G, Devoe C, Nguyen K, Antes A, Krantz A, Rymarczyk G, Wilczynski A, Empig C, Freimark B, Gray M, Schlunegger K, Hutchins J, Kotenko S, Birge R
    Mol Cancer Res, 0;15(6):753-764.
    Applications: ELISA (Standard)

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Reviews for Recombinant Human Gas6 Protein, CF

Average Rating: 4.3 (Based on 4 Reviews)

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Recombinant Human Gas6 Protein, CF
By Anonymous on 01/04/2019
Application: In vitro bioactivity in cell culture

Recombinant Human Gas6 Protein, CF
By Anonymous on 07/09/2018
Application: SDS-PAGE Control

Recombinant Human Gas6 Protein, CF
By Anonymous on 04/03/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human Gas6 Protein, CF
By Anonymous on 04/26/2017
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