Recombinant Human B7-H2 His-tag Protein, CF

(1 Review)
    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL. Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808. The ED50 for this effect is 0.3-1.2 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived human B7-H2 protein
    Asp19-Ser258, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Asp19
  • Predicted Molecular Mass
    28 kDa
  • SDS-PAGE
    59-64 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

8206-B7
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Images
Recombinant Human B7-H2 co-stimulates IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.
1 μg/lane ofRecombinant Human B7-H2 (Catalog # 8206-B7) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing bands at 64.3 and 64.4 kDa, respectively.
Background: B7-H2
B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H2 consists of a 238 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain (2, 3). Within the ECD, human B7-H2 shares 50% and 54% aa sequence identity with mouse and rat B7‑H2, respectively. Alternative splicing generates a long isoform that carries a 10 aa substitution for the C-terminal 3 residues. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells (2-6). It binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation (2, 4, 7). Mouse and human B7-H2 exhibit cross-species binding to ICOS (3, 6). The B7-H2 interaction with ICOS is co-stimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (Tfh) and germinal centers (2-4, 8, 9). In human but not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate both human and mouse naïve T cells and regulatory T cells (Treg) (6). B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung (8, 10, 11). Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity (5, 12, 13). In contrast, its ligation of ICOS on Tfh cells can increase the severity of autoimmune symptoms (9). A soluble form of human B7-H2 is elevated in the circulation of patients with active systemic lupus erythematosus (14).In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones (15).
  • References:
    1. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
    2. Wang, S. et al. (2000) Blood 96:2808.
    3. Yoshinaga, S.K. et al. (2000) Int. Immunol. 12:1439.
    4. Yoshinaga, S.K. et al. (1999) Nature 402:827.
    5. Faget, J. et al. (2012) Cancer Res. 72:6130.
    6. Yao, S. et al. (2011) Immunity 32:729.
    7. Watanabe, M. et al. (2008) J. Immunol. 180:5222.
    8. Wong, S.-C. et al. (2003) Blood 102:1381.
    9. Hu, Y.-L. et al. (2009) J. Immunol. 182:1421.
    10. Kadkhoda, K. et al. (2010) J. Immunol. 184:3780.
    11. Kadkhoda, K. et al. (2011) Int. Immunol. 23:239.
    12. Gajewska, B.U. et al. (2005) J. Immunol. 174:3000.
    13. Akbari, O. et al. (2002) Nat. Med. 8:1024.
    14. Her, M. et al. (2009) Lupus 18:501.
    15. Wang, F. et al. (2012) J. Clin. Immunol. 32:1253.
  • Long Name:
    B7 Homolog 2
  • Entrez Gene IDs:
    23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)
  • Alternate Names:
    B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 28  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag APC-conjugated Antibody

ICFlow IC050A  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PE-conjugated Antibody

ICFlow IC050P 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Biotinylated Antibody

WB BAM050 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Antibody

WB , Flow , AP MAB050R 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PerCP-conjugated Antibody

ICFlow IC050C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 3 (Based on 1 review)

Have you used Recombinant Human B7-H2 His-tag Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Recombinant Human B7-H2 His-tag Protein, CF.

We have 1 review tested in 1 application: Characterization prior to use.

Filter by Ratings
Average
(1)
All Ratings
Filter by Applications
Characterization prior to use
(1)
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
Characterization prior to use Recombinant Human B7-H2 His-tag Protein, CF 8206-B7 Array 8206-B7
Enlarge
Average
 Characterization prior to use Anonymous 02/07/2018
Show
Image Details
Characterization prior to use Recombinant Human B7-H2 His-tag Protein, CF 8206-B7
Characterization prior to use: Recombinant Human B7-H2 His-tag Protein, CF [8206-B7].

Summary

Application Characterization prior to use
Reason for Rating 70% homogenous by SEC

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee