Recombinant Human B7-H3 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1027-B3-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (7)
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Recombinant Human B7-H3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 3-12 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human B7-H3 protein
Human B7-H3
(Leu29-Pro245)
Accession # NP_079516
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu29
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa (monomer)
SDS-PAGE
70-80 kDa, reducing conditions

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1027-B3

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1027-B3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: B7-H3

Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for regulating immune responses (1‑3). Other family members include B7-1, B7-2, B7-H2, PD-L1 (B7-H1), and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% amino acid (aa) sequence identity. The cloned human B7-H3 cDNA encodes a 316 aa type I membrane precursor protein with a putative 28 aa signal peptide, a 217 aa extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a 45 aa cytoplasmic domain. An isoform of human B7-H3 containing a four-Ig-like domain extracellular region has also been identified. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines. B7-H3 expression is also detected on various normal tissues and in some tumor cell lines. Human B7-H3 does not bind any known members of the CD28 family of immunoreceptors. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to costimulate the proliferation of CD4+ or CD8+ T cells. B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (1-3).

References
  1. Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
  2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Long Name
B7 Homolog 3
Entrez Gene IDs
80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)
Alternate Names
B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule

Citations for Recombinant Human B7-H3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Targeting brain lesions of non-small cell lung cancer by enhancing CCL2-mediated CAR-T cell migration
    Authors: H Li, EB Harrison, H Li, K Hirabayash, J Chen, QX Li, J Gunn, J Weiss, B Savoldo, JS Parker, CV Pecot, G Dotti, H Du
    Nature Communications, 2022;13(1):2154.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Delivery of CAR-T cells in a transient injectable stimulatory hydrogel niche improves treatment of solid tumors
    Authors: AK Grosskopf, L Labanieh, DD Klysz, GA Roth, P Xu, O Adebowale, EC Gale, CK Jons, JH Klich, J Yan, CL Maikawa, S Correa, BS Ou, AI d'Aquino, JR Cochran, O Chaudhuri, CL Mackall, EA Appel
    Science Advances, 2022;8(14):eabn8264.
    Species: Human
    Sample Types: Whole Cell
    Applications: Flow Cytometry
  3. Targeting Radiation-Resistant Prostate Cancer Stem Cells by B7-H3 CAR T Cells
    Authors: Y Zhang, L He, A Sadagopan, T Ma, G Dotti, Y Wang, H Zheng, X Gao, D Wang, AB DeLeo, S Fan, R Sun, L Yu, L Zhang, G Wang, S Ferrone, X Wang
    Molecular Cancer Therapeutics, 2021;20(3):577-588.
  4. Proinflammatory cytokines promote TET2-mediated DNA demethylation during CD8 T�cell effector differentiation
    Authors: CC Zebley, HA Abdelsamed, HE Ghoneim, S Alli, C Brown, D Haydar, T Mi, T Harris, MA McGargill, G Krenciute, B Youngblood
    Cell Reports, 2021;37(2):109796.
    Species: Transgenic Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Evaluation of Prognostic Immune Signatures in Patients with Breast, Colorectal and Pancreatic Cancer Receiving Chemotherapy
    Authors: UM Vogl, L Öhler, M Rasic, JM Frischer, M Modak, J Stöckl
    Anticancer Res., 2017;37(4):1947-1955.
    Applications: ELISA (Standard)
  6. Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis
    Authors: BR Yoon, YH Chung, SJ Yoo, K Kawara, J Kim, IS Yoo, CG Park, SW Kang, WW Lee
    J. Biol. Chem, 2016;291(8):4048-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. B7-h3 augments the inflammatory response and is associated with human sepsis.
    Authors: Zhang G, Wang J, Kelly J
    J. Immunol., 2010;185(6):3677-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Recombinant Human B7-H3 Fc Chimera Protein, CF
By David Burnett on 12/01/2017
Application: Enzymatic activity in vitro
Reason for Rating: Worked perfectly in ELISA analysis of antibody production of cell fusions.